Chondroitinase II from Proteus vulgaris

Details Chondroitinase II from Proteus vulgaris Chondroitinase II from Proteus vulgaris

1994-04-22

1996-03-12

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435201, 435202, 435873, 4352521, C12N 924, C12N 112, C12N 926, C12N 928

Patent

active

054985366

ABSTRACT:
A chondroitinase II is isolated from Proteus vulgaris. This enzyme together with chondroitinase I, is useful for selectively and completely disinserting the ocular vitreous body from the neural retina of the eye. The chondroitinase II has the amino acid sequence of SEQ. ID No. 2, an isoelectric point of from about 8.4 to about 8.45, and a molecular weight of 111,772+27 daltons as determined by electrospray and 111,725+20 daltons as determined by laser desorption. Preferrably, the chondroitinase II and chondroitinase I are co-purified chondroitinases which can then be separately eluted from each other.

REFERENCES:
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Yamagata et al., J. of Biol. Chem., 243(7), 1523-35, 1968.

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