Chondroitinase I and chondroitinase II producing mutants of P. v

Details Chondroitinase I and chondroitinase II producing mutants of P. v Chondroitinase I and chondroitinase II producing mutants of P. v

1995-06-07

1999-03-30

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

4352521, 4352523, 435243, 435873, C12N 988, C12N 120, C12N 121

Patent

active

058887989

ABSTRACT:
Mutant Proteus vulgaris strains are provided that, when grown in the absence of an exogenous chondroitinase I and II inducer, produce P. vulgans chondroitinase I and chondroitinase II proteins. The mutants typically produce chondroitinase I and II proteins in the absence of exogenous inducers and in amounts in excess of those produced by wild-type P. vulgaris strains induced with such inducers. Two classes of such mutants, Classes 1 and 2, are disclosed. Class 1 and class 2 mutants differ in the relative amounts of chondroitinases I and II produced when cells are grown in casamino acids--supplemented minimal medium. Additional phenotypic variants that release chondroitinase I protein into the culture medium are provided as well. Also contemplated is a method for producing P. vulgaris chondroitinase I and II proteins. The mutant cells described above are cultured in the absence of a conventional exogenous chondroitinase I and II inducer, after which the cells are harvested and chondroitinase I and II are recovered from the harvested cells. A method for in situ detection of chondroitinase I and II production by bacterial colonies is also provided.

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