Chondroitin lyase enzymes

Details Chondroitin lyase enzymes Chondroitin lyase enzymes

1994-07-08

2000-07-25

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

424 945, 536 232, C12N 988, C12N 1560, A61K 3851

Patent

active

060935634

ABSTRACT:
The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography, hydroxylapatite chromatography, high resolution ion exchange chromatography and size exclusion is outlined. A 77,000.+-.5,000 Dalton protein capable of degrading chondroitin sulfates A and C and a 55,000.+-.2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use are described.

REFERENCES:
patent: 4390628 (1983-06-01), Johansen
patent: 4696816 (1987-09-01), Brown
patent: 5169772 (1992-12-01), Zimmerman et al.
patent: 5292509 (1994-03-01), Hageman
Y.M. Michelacci et al., "A Comparative Study Between a Chondroitinase B and a Chondroitinase AC From Flavobacterium heparinum: Isolation of a Chondroitnase AC-Susceptible dodecasaccharide From Chondroitin Sulphate B", Biochem. J. 151(1) 121-129, Oct. 1975.
K. Gu et al., "Comparison of the Activity of Two Chondroitin AC Lyases on Dermatan Sulfate", Carbohydrate Res. 244(2) 369-377, Jun. 1993.
T. Yamagata et al., "Purification and Properties of Bacterial Chondroitinases and Chondrosulfatases", J. Biol. Chem. 243(7) 1523-1535, Apr. 1968.
Baird and Klagsbrun, "The Fibroblast Growth Factor Family", Annals New York Acadamy of Sciences Nomenclature Meeting Report And Recommendations xi-xvi (1991).
Baker et al., "A Gene Regulating The Heat Shock Response In Escherichia coli Also Affects Proteolysis", Proc. Natl. Acad. Sci., 81:6779-6783 (1984).
Cohen, et al., "Nonchromosomal Antibiotic Resistance In Bacteria: Genetic Transformation Of Escherichia coli By R-Factor DNA", Proc. Natl. Acad. Sci. USA, 69:2110-2114 (1972).
Edman, "Phenylthiohydantoins In Protein Analysis", Ann. N. Y. Acad. Sci., 88:602-610 (1950).
Folkman, et al., "A Heparin-Binding Angiogenic Protein--Basic Fibroblast Growth Factor--Is Stored Within Basement Membrane", Am. J. of Pathol., 130:393-400 (1988).
Folkman and Klagsbrun, "Angiongenic Factors", Science, 235:442-447 (1987).
Forrester, et al., "A Paradigm For Restenosis Based On Cell Biology: Clues For The Development Of New Preventive Therapies", J. Am. Coll. Cardio., 17:758-769 (1991).
Galliher, et al., "Heparinase Production By Flavobacterium Heparinum", Appl. Environ. Microbiol., 41(2):360-365 (1981).
Gu, et al., "Comparison Of The Activity Of Two Chondroitin AC Lyases On Dermatan Sulfate", Carbohydrate Res., 244:369-377 (1993).
Higashiyama, et al., "A Heparin-Binding Growth Factor Secreted By Macrophage-Like Cells That Is Related To EGF," Science 251:936-939 (1991).
Laemmli, "Cleavage Of Structural Proteins During The Assembly Of The Head Of Bacteriophage T4", Nature, 227:680-685 (1970).
Linhardt, et al., "Polysaccharide Lyases", Appl. Biochem. Biotechnol., 12:135-177 (1986).
Linn, et al., "Isolation And Characterization Of Two Chondroitin Lyases From Bacteroides Thetaiotaomicron", J. Bacteriol., 165:859-866 (1985).
Maniatis, et al., Molecular Cloning, A laboratory Manual (1982).
Michelacci, et al., "Isolation And Characterization Of An Induced Chondroitinase ABC From Flavobacterium Heparinum", Biochimica et Biophysica Acta, 923:291-013 (1987).
Miller, Experiments in Molecular Genetics, Cold Spring Harbor, 1972.
Nathan and Sporn, "Cytokines in Context", J. of Cell Biol., 113:981-986 (1991).
Richardson and Hatton, "Transient Morphological And Biochemical Alterations Of Arterial Proteoglycan During Early Wound Healing", Exp. Mol. Pathol., 58:77-95 (1993).
Sasisekharan, et al., "Heparinase Inhibits Neovascularization", Proc. Natl. Acad., Sci. USA 91:1524-1528 (1994).
Silhavy et al., in Experiments With Gene Fusions, Cold Spring Harbor Laboratory, 1984.
Southern, "Detection Of Specific Sequences Among DNA Fragments Separated By Gel Electrophoresis", J. Mol. Biol., 98:503-517 (1975).
Tabas, et al., "Lipoprotein Lipase And Sphingomyelinase Synergistically Enhance The Association Of Atherogenic Lipoproteins With Smooth Muscle Cells And Extracellular Matrix", J. Biol. Chem., 268(27):20419-20432 (1993).
Takegawa, et al., "Purification And Properties Of Chondroitinase Produced By A Bacterium Isolated From Soil", J. Ferm. Bioeng., 77(2):128-131 (1991).
Vlodaysky, et al., "Endothelial Cell-Derived Basic Fibroblast Growth Factor: Synthesis And Deposition Into Subendothelial Extracellular Matrix", Proc. Natl. Acad. Sci. USA, 84:2292-2296 (1987).
Voss et al., "Automated DNA Sequencing System Resolving 1,000 Bases With Fluorescein-15-*dATP As Interanl Label", Meth. Molec. Cell. Biol., 3:153-155 (1992).
Werner, et al., "Large Induction Of Keratinocyte Growth Factor Expression In The Dermis During Would Healing", Proc. Natl. Acad. Sci. USA, 89:6896-6900 (1992).
Yamagata, et al., "Purification And Properties Of Bacterial Chaondroitinases And Chondrosulfatases", The Journal Bio. Chemistry, 243(7):1523-1535 (1968).
Yang, et al., "Purification And Characterization Of Heparinase from Flavobacterium Heparinum", J. Biol. Chem., 160(30):1849-1857 (1985).
Yeo, et al., "Alterations In Proteoglycan Synthesis Common To Healing Wounds And Tumors", Am. J. Pathol., 138:1437-1450 (1991).

Affiliated with

Also associated with

Rating

Chondroitin lyase enzymes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chondroitin lyase enzymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chondroitin lyase enzymes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1335582