Cholesterol disposal fusion enzymes

Details Cholesterol disposal fusion enzymes Cholesterol disposal fusion enzymes

1993-06-09

1996-08-20

Walsh, Stephen G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435188, 530401, C12N 902, C07K 1480

Patent

active

055478689

ABSTRACT:
Fusion enzymes having multiple segments of different biological activity including one segment having P450scc activity and at least one segment having electron-transfer activity for transferring electrons to P450scc are described along with genetic constructs for production of such enzymes and methods for their use. Methods of their use include cholesterol degradation in vitro or in vivo as well as conversion of cholesterol to other useful steroidal products including pregnenolone.

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