Cholesterol 7 .alpha.-hydroxdylase gene regulatory elements and

Details Cholesterol 7 .alpha.-hydroxdylase gene regulatory elements and Cholesterol 7 .alpha.-hydroxdylase gene regulatory elements and

1994-01-28

1998-05-19

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4351723, 4352402, 4353201, 536 241, C12Q 168, C12N 510, C12N 1511, C12N 1585

Patent

active

057534314

ABSTRACT:
DNA regulatory elements that control cholesterol 7.alpha.-hydroxylase expression are disclosed, including bile acid responsive elements. A gene construct comprising at least one CYP7 regulatory element and a reporter gene is used to transfect HepG2 cells. Confluent transfected HepG2 cells are employed in an assay to detect a compound that modulates cholesterol 7.alpha.-hydroxylase enzyme regulation. A method for screening compounds that inhibit or stimulate expression of the enzyme is provided, as well as a method for detecting and isolating transcription factors of the cholesterol 7.alpha.-hydroxylase gene. A transcription factor of 57 KDa is identified which is useful in an assay for determining regulation of CYP7 expression.

REFERENCES:
patent: 5420028 (1995-05-01), Chiang
patent: 5558999 (1996-09-01), Chiang
Ness et al. "Effect of Thyroid Hormone on Hepatic Cholesterol 7.alpha.Hydroxylase, LDL Receptor, HMG-CoA Reductase, Farnesyl Pyrophosphate Synthetase and Apolipoprotein A-I mRNA Levels in Hypophysectomized Rats", Biochem. and Biophys. Res. Comm., 172(3):1150-1156, (1990).
Lusis, Aldons J., "The Mouse Model for Atherosclerosis", TCM 3(4): 1335-143 (1993).
Dueland, Svein et. al., "Effect of Dietary Cholesterol and Taurocholate on Cholesterol 7.alpha.-hydroxylase and Hepatic LDL Receptors in Inbred Mice", Journal of Lipid Research 34: 923-931 (1993).
Dueland, Svein et. al., "Expression of 7.alpha.-Hydroxylase in Non-hepatic Cell Results in Liver Phenotypic Resistance of the Low Density Lipoprotein Receptor to Cholesterol Repression", Journal of Biological Chemistry 267(32): 22695-22698 (1992).
Karam et al. "Polymorphisms of Human Cholesterol 7.alpha.-Hydroxylase", Biochem. and Biophys. Res. Comm. 185(2): 588-595 (1992).
Breslow et al. "Transgenic Mouse Models of Lipoprotein Metabolism and Atherosclerosis", Proc. Natl. Acad. Sci. USA 90: 8314-8318 (1993).
Cohen et al. "Cloning of the Human Cholesterol 7.alpha.Hydroxylase Gene (CYP7) and Localization to Chromosome 8q11-812", Genomics 14: 153-161 (1992).
Nishimoto et al. "Structure of the Gene Encoding Human Liver Cholesterol 7.alpha.-Hydroxylase", Biochimica. et Biophysica. Acta. 1172: 147-150 (1992).
Thompson et al. "Cholesterol 7.alpha.-Hydroxylase Promoter Separated from Cyclophilin Pseudogene By Alu Sequence", Biochimica et Biophysica Acta 1168: 239-242 (1993).
Li et al. "The Expression of a Catalytically Active Cholesterol 7.alpha.-Hydroxylase Cytochrome P450 in Escherichia coli", The Journal of Biological Chemistry 266(29): 19186-19191 (1991).
Molowa et al. "Transcriptional Regulation of the Human Cholesterol 7.alpha.-Hydroxylase Gene", Biochemistry 31: 2539-2544 (1992).
Nishimoto et al. "Structural Analysis of the Gene Encoding Rat Cholesterol .alpha.-Hydroxylase, The Key Enzyme for Bile . . . ", The Journal of Biological Chemistry 266(10): 6467-6471 (1991).
Jelinek et al. "Structure of the Rat Gene Encoding Cholesterol 7.alpha.-Hydroxylase", Biochemistry 29(34): 7781-7785 (1990).
Chiang et al. "Cloning and 5'-Flanking Sequence of a Rat Cholesterol 7.alpha.-Hydroxylase", Biochimica et Biophysica Acta 1132: 337-339 (1992).
Crestani et al. "Genomic Cloning, Sequencing, and Analysis of the Hamster Cholesterol 7.alpha.-Hydroxylase Gene (CYP7).sup.1 ", Archives of Biochem. and Biophy., 306(2): 451-460 (1993).
Ausubel et al. (1992), Short Protocols in Molecular Biology, Second Edition, (John Wiley & Sons, New York), pp. 9-17 to 9-23.
Lai et. al. (1991) TIBS 16, 427-430.
Sambrook et al. (1989) "Molecular Cloning: A Laboratory Manual, Second Ed." pp. 15.3-15.4, 15.14-15.19, 15.32-15.36, 15.51-15.52 CHS Press N.Y.
Wasylyk (1988) Biochem. Biophys. ACTA 951, 17-35.
Hylemon et al. (1992) J. Biol. Chem. 267(24), 16866-16871.
Pandak et al. (1991) J. Biol. Chem. 266(6), 3416-3412.
Pandak et. al. (1992) J. Lipid Res. 33, 659-668.
Crestani et al. (1992) FASEB J 6(4) A2626.

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