CHO cells that express human LH-RH receptor

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Rodent cell – per se

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435358, C12N 1585

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active

056771843

ABSTRACT:
Disclosed are CHO cells which are capable of continued production of human LH-RH receptor proteins, or cell membrane fractions thereof; recombinant human LH-RH receptor proteins or partial peptides thereof; methods for screening compounds which have affinity for an LH-RH receptor by contacting the compound with the CHO cells or the cell membrane fractions thereof, or the recombinant human LH-RH receptor proteins or the partial peptides thereof; kits for screening them; the compounds which have affinity for the LH-RH receptor obtained by methods for the screening or kits for the screening; and pharmaceutical compositions containing the compound, thereby being able to early provide prophylactic or therapeutic compositions, for example, for prostate cancer, uterine cancer, breast cancer, a pituitary tumor, endometriosis, hysteromyoma or precocious puberty. They are also useful as a pregnancy controlling composition such as contraceptive or a menstrual cycle controlling composition.

REFERENCES:
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Kakar et al. BBRC (1992) 189: 289-295.
Chi et al. Mol. Cell. Endocrinal. (1993) 91:1-6.
Yamauchi et al. Biosci. Biotech Biochem (1992) 56: 600-604.
Costagliola et al. J. Clin. Endocrin. Metab. (1992) 75:1540-1544.
Nagayama et al. Biochem. Biophys. Res. Comm. 165(1989) 1184-1190.

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