Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1997-06-09
1999-07-27
Ketter, James
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435200, 435358, 536 232, C12N 1564, C12N 1556
Patent
active
059289155
ABSTRACT:
A recombinant cell line has a constitutive sialidase whose functional expression is disrupted, for example by homologous recombination or using antisense RNA. Sialidase is purified from cell culture fluid of Chinese hamster ovary cells. DNA encoding sialidase is obtained using an oligonucleotide probe designed using amino acid sequence data on the sialidase, and the DNA is expressed in host cells transformed with the DNA.
REFERENCES:
patent: 4912040 (1990-03-01), Kaufman et al.
patent: 5047335 (1991-09-01), Paulson et al.
patent: 5075218 (1991-12-01), Jette et al.
Ferrari et al., "Cloning and Expression of a Soluble Sialidase from Chinese Hamster Ovary Cells: Sequence Alignment Similarities to Bacterial Sialidases" Glycobiology 4(3):367-373 (Jun. 1994).
Godoy et al., "A Role for Bacteroides Fragilis Neuraminidase in Bacterial Growth in Two Model Systems" Infect. Immun. 61(10):4415-4426 (Oct. 1993).
Gramer et al., "Potential for Degradation of Glycoprotein Oligosaccharides by Extracellular Glycosidases" Am. Chem. Soc. (Abstract, 203rd Meeting, San Francisco, CA) PT 1:BIOT-71 (Apr. 5, 1992).
Miyagi et al., "Multiple Forms of Mammalian Sialidase: Altered Expression in Carcinogenesis" Tohoku J. Exp. Med. 168:223-229 (1992).
Sheehan et al., "A Single Amino Acid Substitution in the Hemagglutinin-neuraminidase of Newcastle Disease Virus Results in a Protein Deficient in Both Functions" Virology 189(2):778-781 (Aug. 1992).
Stanley, P., "CHO Mutants Available Glycosylation Engineering" (Abstract from conference on Glycotechnology held in San Francisco May 17-19) (1993).
Warner et al., "Isolation and Properties of a Soluble Sialidase from the Culture Fluid of Chinese Hamster Ovary Cells" Glycobiology 3(5):455-463 (Oct. 1993).
Waxham et al., "Identification of Amino Acids Involved in the Sialidase Activity of the Mumps Virus Hemagglutinin-neuraminidase Protein" Virology 167(1):226-232 (Nov. 1988).
Sambrook et al., Molecular Cloning: A Laboratory Manual, 2.sup.nd ed., Cold Spring Harbor Lab. Press, 1989, pp. 12.2-12.29.
Miyagi et al., J. Biochem., vol. 107, 1990, pp. 794-798.
Miyagi et al., Tohoku J. Exp. Med., vol. 168, 1992, pp. 223-229.
Burger et al., J. General Virology, vol. 72, 1991, pp. 359-367.
Air et al., "The Neuraminidase of Influenza Virus", Proteins: Structure, Function, Genetics 6:341-356 (1989).
Miyagi et al., "Purification and Characterization of Cytosolic Sialidase from Rat", The Journal of Biological Chemistry, vol. 260, No. 11, Issue of Jun. 10, pp. 6710-6716, 1985.
Schengrund et al., Ecto-Gangliosidase Activity of Herpes Simplex Virus-Transformed Hamster Embryo Fibroblasts, The Journal of Cell Biology, vol. 70, pp. 555-561, 1976.
Tulsiani et al., "Studies on the Soluble and Lysosomal Neuraminidases of Rat Liver", The Journal of Biological Chemistry, vol. 245, No. 7, Isue of Apr. 10, pp. 1821-1827, 1970.
Sliwkowski Mary B.
Warner Thomas G.
Genentech Inc.
Johnston Sean A.
Ketter James
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