Chlamydia assay using amidine modified supports or particles

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 732, 435 14, 435 19, 435 21, 435 28, 436510, 436518, 436534, 436538, 436541, 436823, G01N 33571

Patent

active

050305611

ABSTRACT:
A method for assaying of Chlamydia includes adhering Chlamydia antigen to amidine modified latex particles, binding of adhered antigen to an anti-Chlamydia antibody conjugated to an enzyme, separating the particles from the liquid phase of the assay and detecting bound enzyme by color development when the separated particles are contacted with a substrate for the enzyme. The invention includes a kit of materials for preforming an assay in accordance with the method of the invention.

REFERENCES:
patent: 4477578 (1984-10-01), Miles et al.
patent: 4497899 (1985-02-01), Armstrong et al.
patent: 4511662 (1985-04-01), Baran et al.
patent: 4888276 (1989-12-01), Shelburne
Kricker, Ligand-Binder Assays, Chap. 3, Separation Procedures, Marcel Dekker, Inc. 1985, pp. 53-65.
Baker, et al., Immunoradiometric Assays, Chap. 5, Alternative Immunoassays, 1985, pp. 59-68.

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