Chitosan core matrix containing cells encapsulated in a thermopl

Details Chitosan core matrix containing cells encapsulated in a thermopl Chitosan core matrix containing cells encapsulated in a thermopl

1999-02-16

2000-10-31

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

424 937, 435182, 435382, C12N 1110, C12N 1104, C12N 500

Patent

active

061400890

ABSTRACT:
Encapsulated viable cells for implanting are prepared having cells dispersed in a particulate, essentially non cross-linked chitosan core matrix that is enclosed within a semipermeable membrane. The cells are entrapped between chitosan particles of the core matrix and there is essentially no interfacial cross-linking between the core matrix and the membrane. The core matrix provides a physical support for the cells such that the cells are evenly dispersed throughout the core matrix so as to allow their maintenance, growth, proliferation and differentiation. The encapsulated cells may be prepared by mixing viable cells with a solution of chitosan, encapsulating the resultant mixture in a thermoplastic semipermeable membrane, and causing the chitosan to precipitate such as by changing the pH to form the core matrix. Alternatively, the chitosan in solution is precipitated to form the core matrix containing cells, and the core matrix is encapsulated in a semipermeable membrane. Cells encapsulated include neurosecretory cell lines, .beta.-cell-derived cells lines, fibroblasts, myocytes and glial cells.

REFERENCES:
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patent: 4892538 (1990-01-01), Aebischer et al.
patent: 5158881 (1992-10-01), Aebischer et al.
patent: 5871985 (1999-02-01), Aebischer et al.

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