Chimeric West Nile viruses and uses thereof

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Recombinant virus encoding one or more heterologous proteins...

Reexamination Certificate

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C424S218100, C424S204100, C424S205100, C435S235100, C435S320100, C435S325000, C536S023720, C514S04400A

Reexamination Certificate

active

07455842

ABSTRACT:
A chimeric West Nile live virus comprised of the NY99 and WN1415 strains and an infectious DNA recombinant construct encoding for the chimeric virus are provided, as well as immunogenic compositions and their method of use.

REFERENCES:
patent: 7094411 (2006-08-01), Kinney et al.
patent: 2005/0163804 (2005-07-01), Chang
patent: 2005/0276816 (2005-12-01), Yamshchikov
patent: WO 9306214 (1993-04-01), None
Beasley et al., “Envelope Glycoprotein Glycosylation Status Influences Mouse Neuroinvasion Phenotype of Genetic Lineage 1 West Nile Virus Strains,” Journal of Virology, vol. 79, No. 13 (Jul. 2005).
Petersen et al., “West Nile Virus: A Reemerging Global Pathogen,” Emerging Infectious Diseases, vol. 7, No. 4 (2001).
Yamshchikov, et al., (2004). An attenuated West Nile prototype virus is highly immunogenic and protects against the deadly NY99 strain: a candidate for live WN vaccine development.Virology 330, 304-312.
Yamshchikov, et al., (2001a). A new strategy in design of +RNA virus infectious clones enabling their stable propagation inE. coli. Virology 281, 272-280.
Yamshchikov, et al., (2001b). An infectious clone of the West Nile flavivirus.Virology 281, 294-304.

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