Chimeric toxins

Details Chimeric toxins Chimeric toxins

1998-03-30

1999-01-26

Hendricks, Keith D.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

514 12, 530350, 530351, 435194, C12N 912, A61K 3816

Patent

active

058638911

ABSTRACT:
A chimeric toxin comprising protein fragments joined together by peptide bonds, the chimeric toxin comprising, in sequential order, beginning at the amino terminal end of the chimeric toxin,
(a) the enzymatically active Fragment A of diphtheria toxin,
(b) a first fragment including the cleavage domain 1.sub.1 adjacent the Fragment A of diphtheria toxin,
(c) a second fragment comprising at least a portion of the hydrophobic transmembrane region of Fragment B of diphtheria toxin, the second fragment having a deletion of at least 50 diphtheria toxin amino acid residues, the deletion being C-terminal to the portion of the transmembrane region, and the second fragment not including domain 1.sub.2, and
(d) a third fragment comprising a portion of a cell-specific polypeptide ligand, the portion including at least a portion of the binding domain of the polypeptide ligand, the portion of the binding domain being effective to cause the chimeric toxin to bind selectively to a predetermined class of cells to be attacked by the enzymatically active Fragment A.

REFERENCES:
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patent: 4830962 (1989-05-01), Gelfand et al.
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