Chimeric retroviral gag genes and screening assays

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C530S300000, C435S005000, C435S069100, C424S204100, C424S207100, C424S208100

Reexamination Certificate

active

07148325

ABSTRACT:
The subject invention provides novel and advantageous methods for identifying amino acid sequences in random peptide libraries that can bind to Gag polypeptides. The subject invention also establishes a novel in vitro system that can be used to test competitive inhibitors of retrovrial capsid assembly. Also provided are peptides, and compositions containing these peptides, which are inhibitors of the retrovirus Gag protein(s) function. Chimeric Gag polypeptides are also provided.

REFERENCES:
patent: 4816567 (1989-03-01), Cabilly
patent: 4918166 (1990-04-01), Kingsman et al.
patent: 0 803 574 (1997-10-01), None
Sakalian J Virology 1996, vol. 70, pp. 3706-3715.
Sakalian and Hunter 1999 J of Virology vol. 73, pp. 8073-8082.
Ausubel, F.M. et al.,Current protocols in Molecular Biology, 1989, John Wiley and Sons, New York City. (Book).
Bennett, R. P., Nelle, T. D., and Wills, J. W., “Functional chimeras of the Rous sarcoma virus and human immunodeficiency virus gag proteins,”Journal of Virology, 1993, p. 6487-6498, vol. 67, No. 11.
Berkowitz, R., Fisher, J., and Goff, S. P., “RNA packaging,”Current Topics in Microbiology and Immunology, 1996, p. 177-218, vol. 214.
Berkowitz, R. D., Luban, J., and Goff, S. P., “Specific binding of human immunodeficiency virus type 1gagpolyprotein and nucleocapsid protein to viral RNAs detected by RNA mobility shift assays,”Journal of Virology, 1993, p. 7190-7200, vol. 67 No. 12.
Blake, J., Johnston, J. V., Hellström, K. E., Marquardt, H., and Chen, L., “Use of combinatorial peptide libraries to construct functional mimics of tumor epitopes recognized by MHC class I-restricted cytolytic T lymphocytes,”J. Exp. Med., 1996, p. 121-130, vol. 184.
Bolognesi, D. P., Luftig, R., and Shaper, J. H., “Localization of RNA tumor virus polypeptides. I. Isolation of further virus substructures,”Virology, 1973, p. 549-564, vol. 56.
Böttcher, B., Wynne, S. A., and Crowther, R. A. “Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy,”Nature, 1997, p. 88-91, vol. 386.
Bryant, M., and Ratner, L., “Myristoylation-dependent replication and assembly of human immunodeficiency virus 1,”Proc. Natl. Acad. Sci. USA1990, p. 523-527, vol. 87.
Campbell, S., and Vogt, V. M., “Self-assemblyin vitroof purified CA-NC proteins from Rous sarcoma virus and human immunodeficiency virus type 1,”Journal of Virology, 1995, p. 6487-6497, vol. 69, No. 10.
Campbell, S., and Vogt, V. M., “In vitroassembly for virus-like particles with Rous sarcoma virus gag deletion mutants: Identification of the p10 domain as a morphological determinant in the formation of spherical particles,”Journal of Virology, 1997, p. 4425-4435, vol. 71, No. 6.
Chien, C. T., Bartel, P. L., Sternglanz, R., and Fields, S., “The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest,”Proc. Natl. Acad. Sci. USA, 1991, p. 9578-9582, vol. 88.
Clackson, T., Hoogenboom, H. R., Griffiths, A. D., and Winter, G., “Making antibody fragments using phage display libraries,”Nature, 1991, p. 624-628, vol. 352.
Coffin, J. M., Hughes, S. H., and Varmus, H. E., eds.,Retroviruses, 1997, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. (Book).
Conte, M. R., Klikova, M., Hunter, E., Ruml, T., and Matthews, S., “The three-dimensional solution structure of the matrix protein from the type D retrovirus, the Mason-Pfizer Monkey virus, and implications for the morphology of retroviral assembly,”Embo J., 1997, p. 5819-5826, vol. 16, No. 19.
Conway, J. F., Cheng, N., Zlotnick, A., Wingfield, P. T., Stahl, S. J., and Steven, A. C., “Visualization of a 4-helix bundle in the hepatitis B virus capsid by cryo-electron microscopy,”Nature, 1997, p. 91-94, vol. 386.
Craven, R. C., and Parent, L. J., “Dynamic interactions of the Gag polyprotein,”Current Topics in Microbiology and Immunology, 1996, p. 65-94, vol. 214.
Delchambre, M., Gheysen, D., Thines, D., Thiriart C., Jacobs, E., Verdin, E., Horth, M., Burny, A., and Bex, F., “The Gag precursor of simian immunodeficiency virus assembles into virus-like particles,”EMBO J., 1989, p. 2653-2660, vol. 8, No. 9.
Dickson, C., Eisenman, R., Fan, H., Hunter, E., and Teich, N., “Protein biosynthesis and assembly,”RNA tumor viruses, R. Weiss, N. Teich, H. Varmus, and J. Coffin, eds., 1984, p. 513-648, vol. 1, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Eisenman, R., Vogt, V. M., and Diggelmann, H., “Synthesis of avian RNA tumor virus structural proteins,”Cold Spring Harbor Symp. Quant. Biol., 1974, p. 1067-1075, vol. 39.
Fields, S., and Song, O., “A novel genetic system to detect protein-protein interactions,”Nature, 1989, p. 245-246, vol. 340.
Fields, S., and Sternglanz, R., “The two-hybrid system: an assay for protein-protein interactions,”Trends Genet., 1994, p. 286-292, vol. 10, No. 8.
Garnier, L., Wills, J. W., Verderame, M. F., and Sudol, M., “WW domains and retrovirus budding,”Nature, 1996, p. 744-745, vol. 381.
Gebhardt, A., Bosch, J. V., Ziemiecki, A., and Friis, R. R., “Rous sarcoma virus p19 and gp35 can be chemically crosslinked to high molecular weight complexes: An insight into virus assembly,”J. Mol. Biol., 1984, p. 297-317, vol. 174.
Gelderblom, H., “Morphogenesis, maturation, and fine structure of lentiviruses,”Retroviral Proteases: Control of Maturation and Morphogenesis, L. H. Pearl, ed., 1990, p. 159-180, Stockton Press, New York, NY. (Book).
Gelderblom, H. R., “Assembly and morphology of HIV: potential effect of structure on viral function,”AIDS, 1991, p. 617-638, vol. 5.
Gelderblom, H. R., Hausmann, E. H. S., Özel, M., Pauli, G., and Koch, M. A., “Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins,”Virology, 1987, p. 171-176. vol. 156.
Gheysen, D., Jacobs, E., De Foresta, F., Thiriart, C., Francotte, M., Thines, D., and De Wilde, M., “Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cells,”Cell, 1989, p. 103-112. vol. 59.
Gitti, R. K., Lee, B. M., Walker, J., Summers, M. F., Yoo, S., and Sundquist, W. I., “Structure of the amino-terminal core domain of the HIV-1 capsid protein,”Science, 1996, p. 231-235, vol. 273.
Goding, J.W.,Monoclonal Antibodies: Principles and Practice, 1983, Academic Press, New York. (Book).
Gonzalez, S. A., Affranchino, J. L., Gelderblom, H. R., and Burny, A., “Assembly of the matrix protein of simian immunodeficiency virus into virus-like particles,”Virology, 1993, p. 548-556, vol. 194.
Gorelick, R. J., Nigida, S. M., Bess, J. W., Arthur, L. O., Henderson, L. E., and Rein, A., “Non-infectious human immunodeficiency virus type 1 mutants deficient in genomic RNA,”Journal of Virology, 1990, p. 3207-3211, vol. 64, No. 7.
Gorelick, R. J., Henderson, L. E., Hanser, J. P., and Rein, A., “Point mutants of Moloney murine leukemia virus that fail to package viral RNA: Evidence for specific RNA recognition by a “zinc-finger-like” protein sequence,”Proc. Natl. Acad. Sci. USA, 1988, p. 8420-8424, vol. 85.
Göttlinger, H. G., Dorfman, T., Sodroski; J. G., and Haseltine, W. A., “Effect of mutations affecting the p6gagprotein on human immunodeficiency virus particle release,”Proc. Natl. Acad. Sci. USA, 1991, p. 3195-3199, vol. 88.
Göttlinger, H. G., Sodroski, J. G., and Haseltine, W. A., “Role of capsid precursor processing and myristoylation in morphogenesis and infectivity of human immunodeficiency virus type 1,”Proc. Natl. Acad. Sci. USA, 1989, p. 5781-5785, vol. 86.
Haffar, O., Garrigues, J., Travis, B., Moran, P., Zarling, J., and Hu, S. -L., “Human immunodeficiency virus-like, non-rep

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