Chimeric retrovial expression vectors and particles containing a

Details Chimeric retrovial expression vectors and particles containing a Chimeric retrovial expression vectors and particles containing a

1996-04-03

1998-06-23

Achutamurthy, Ponnathapura

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435236, 4351723, 4353201, 4241991, 4242071, 4242081, C12N 700, C12N 701, C12N 1500, C12N 1509

Patent

active

057704287

ABSTRACT:
This invention is directed toward novel replication competent retroviral expression vectors and particles containing a single chimeric viral genome terminal repeat (LTR), complex retroviral (e.g., Bovine Leukemia Virus regions, complex retroviral cis-acting sequences (e.g., the encapsidation sites), and an RNA translational enhancer containing an internal ribosome entry site (IRES). These chimeric viral genomes express the BLV or HIV gag, pol, and env genes in a tax/rex- or tat-/rev-independent manner, respectively. Moreover, the inclusion of an IRES site in the modified viral genome facilitates the expression of the env gene products. These retroviral vectors and particles provide a source of viral antigens that can be employed in both diagnostic assays and as immunogens for the production of specific immunological reagents.

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