Chemistry: molecular biology and microbiology – Vector – per se
Patent
1998-03-11
2000-02-15
Wax, Robert A.
Chemistry: molecular biology and microbiology
Vector, per se
435 697, 530350, 536 234, C12N 1500, C12P 2104, C07K 100, C07H 2104
Patent
active
060251964
ABSTRACT:
HNF-4 (hepatocyte nuclear factor 4) is a protein enriched in liver extracts that binds to sites required for the transcription of the transthyretin (TTR) and apolipoprotein CIII (apoCIII) genes (Costa et al., 1989; Costa et al., 1990; Leff et al., 1989). We have purified HNF-4 protein (54 kD) and isolated a cDNA clone encoding the protein. HNF-4 is a member of the steroid hormone receptor superfamily with an unusual amino acid in the conserved "knuckle" of the first zinc finger (DGCKG). This and the fact that HNF-4 does not bind significantly to estrogen, thyroid hormone or glucocorticoid response elements indicate that HNF-4 may represent a new subfamily. HNF-4 binds to its recognition site as a dimer and activates transcription in a sequence-specific fashion in nonhepatic (HeLa) cells. HNF-4 mRNA is present in kidney and intestine as well as liver but is absent in other tissues. DNA binding data suggest that HNF-4 could be identical to liver factor A1 (LF-A1), a factor previously shown to regulate the transcription of the .alpha.-1 antitrypsin, apolipoprotein A1 and pyruvate kinase genes.
REFERENCES:
patent: 4394443 (1983-07-01), Weissman et al.
patent: 5310662 (1994-05-01), Evans et al.
patent: 5599904 (1997-02-01), Evans et al.
Ahe, von der D., Janich, S., Scheidereit, C., Renkawitz, R., Schutz, G., and Beato, M. (1985). Glucocorticoid and progesterone receptors bind to the same sites in two hormonally regulated promoters. Nature, 313, 706-709.
Baumhueter, S., Mendel, D. B ., Conley, P. B., Kuo, C. J., Turk, C., Graves, M. K., Edwards, C. A., Courtois, G., and Crabtree, G. R. (1990). HNF-1 shares three sequence motifs with the POU domain proteins and is identical to LF-B1 and APF. Genes and Development 4, 372-379.
Beato, M. (1989). Gene regulation by steroid hormones. Cell 56, 335-344.
Bell, J. Ellis, 1988, Protein Purification: Affinity Chromatography. Proteins and Enzymes Prentice-Hall Inc.. 45-63.
Birkenmeier, E.H., Gwynn, B., Howard, S., Jerry, J., Gordon, J.I., Landschulz, W.H., and McKnight, S.L. (1989). Tissue-specific expression, developmental regulation and mapping of the gene encoding CCAAT/enhancer binding protein. Genes and Development, 3, 1146-1156.
Brand, N., Petkovich,M., Krust, A., Chambon, P., de The, H., Marchio, A., Tiollais, P., and Dejean, A. (1988). Identification of a second human retinoic acid receptor. Nature, 332, 850-853.
Briggs, M.R., Kadonaga, J.T., Bell, S.P., and Tjian, R. (1986) Purification and biochemical characterization of the promoter-specific transcription factor, Sp1. Science, 234, 47-52.
Breslow, J. (1988). Apolipoprotein genetic variation and human disease. Physiol. Reviews, 68, 85-132.
Capon, D. J. et al. (1989). Designing CF4 immunoadhesins for AIDS therapy. Nature, 337, 525-531.
Carlsson, R., and Glad, C. (Jun., 1989). Monoclonal antibodies into the '90s. Bio/Technology, 7, 567-573.
Cate, R. et al. (1986). Isolation of the bovine and human genes for Mullerian inhibiting substance and expression of the human gene in animal cells. Cell, 45, 685-598.
Cech, T. R. (1988). Ribozymes and their medical implications. J. Amer. Med. Assn., 260, 3030-3044.
Chomezynski, P. and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
Costa, R. H., Lai, E., and Darnell, J. E., Jr. (1986). Transcriptional control of the mouse prealbumin (transthyretin) gene: both promoter sequences and a distinct enhancer are cell specific. Mol. and Cell. Biol., 6, 4697-4708.
Costa, R. H., Grayson, D. R., Xanthopoulos, K.G., and Darnell, J. E., Jr. (1988). A liver-specific DNA-binding protein recognizes multiple nucleotide sites in regulatory regions of transthyretin, a1-antitrypsin, albumin, and simian virus 40 genes. Proc. Natl. Acad. Sci., 85, 3840-3844.
Costa, R. H., Grayson, D. R. and Darnell, J. E. Jr. (1989). Multiple hepatocyte-enriched nuclear factors function in the regulation of transthyretin and a1-antitrypsin genes. Mol. and Cell. Biol., 9, 1415-1425.
Costa, R. H., Van Dyke, T. A., Yan, C., Kuo, F., and Darnell, J. E., Jr. (1990). Similarities in transthyretin gene expression and differences in transcription factors: liver and yolk sac compared to choroid plexus. Proc. Natl. Acad. Sci. USA, 87, 6589-6593.
Courtois, G., Morgau, J. G., Campbell, L. A., Fourel, G. and Crabtree, G. R. (1987). Interaction of a liver-specific nuclear factor with the fibrinogen and al-antitrypsin promoters. Science, 238, 688-692.
Danielsen, M., Hinck, L., and Ringold, G. M. (1989). Two amino acids within the knuckle of the first zinc finger specify DNA response element activation by the glucocorticoid receptor. Cell, 57, 1131-1138.
Davis, M. M. (1986). Subtractive cDNA hybridization and the T-cell receptor gene. Handbook of Experimental Immunology in Four Volumes, 4th ed. Blackwell Scientific Publications, Oxford, England, 76.1-76.13.
Davis, M. M. et al. (1984). Cell type-specific cDNA probes and the murine I region: The localization and orientation of ad. Proc. Natl. Acad. Sci. USA, 81, 2194-2198.
Derman, E., Krauter, K., Walling, L., Weinberger, C., Ray, M. and Darnell, J. E. Jr. (1981). Transcriptional control in the production of liver-specific mRNAs. Cell, 23, 731-739.
de The, H., Marchio, A., Tiollais, P., and Dejean, A. (1987). A novel steroid thyroid hormone receptor-related gene inappropriately expressed in human hepatocellular carcinoma. Nature, 330, 667-670.
Duguid, J. R. et al. (1988). Isolation of cDNAs of scrapie-modulated RNMAs by subtractive hybridization of a cDNA library. Proc. Natl. Acad. Sci. USA, 85, 5738-5742.
Evans, R.M. (1988). The steroid and thyroid hormone receptor superfamily. Science, 240, 889-895.
Fawell, S.E., Lees, J.A., White,R. and Parker, M.G. (1990). Characterization and colocalization of steroid binding and dimerization activities in the mouse estrogen receptor. Cell, 60, 953-962.
Feinberg, A.P., and Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem., 132, 6-13.
Fisher, R. A. et al. (1988). HIV infection is blocked in vitro by recombinant soluble CD4. Nature, 331, 76-78.
Forman, B. M., Yan, C. R., Au, M., Casanova, J., Ghysdael, J., and Samuels, H. H. (1989). A domain containing leucine-zipper-like motifs mediate novel in vivo interactions between the thyroid hormone and retinoic acid receptors. Mol. End., 3, 1610-1626.
Forman, B. M. and Samuels, H. H. (1990). Interactions among a subfamily of nuclear hormone receptors: The regulatory zipper model. Mol. End. 4, 1293-1301.
Frain, M., Swart, G., Monaci, P., Nicosia, A., Stampfli, S., Frank, R., and Cortese, R. ( 1989). The liver-specific transcription factor LF-Bl contains a highly diverged homeobox DNA binding domain. Cell, 59, 145-157.
Fried, M. and Crothers, D. M. (1981). Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res., 9, 6505-6525.
Giguere, V., Yang, N., Segui, P., and Evans, R. M. (1988). Identification of a new class of steroid hormone receptors. Nature, 331, 91-94.
Glass, C.K. Holloway, J.M., Devary, O.V., and Rosefeld, M. (1988) The thyroid hormone receptor binds with opposite transcription effects to a common sequence motif in thyroid hormone and estrogen response elements. Cell, 54:313-323.
Glass, C. K., Lipkin, S. M., Devary, O. V., and Rosenfeld, M. G. (1989). Positive and negative regulation of gene transcription by a retinoic acid-thyroid hormone receptor heterodimer. Cell, 59, 697-708.
Gorman, C. M., Moffat, L. F. and Howard, B. H. (1982). Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol., 2, 1044-1051.
Gorman, C. M., Howard, B. H. and Reeves, R. (1983). Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate. Nucleic Acids Res., 11, 7631-7648.
Gorski, K., Carneiro, M. and Schibler, U. ( 1986). Tissue-specific in vitro transcription from the mouse albumin promoter. Cell, 47, 767-776.
Green, N., Alexander, H., Olson, A., Alexander, S., Shinnick, T.M
Darnell, Jr. James E.
Sladek Frances M.
Zhong Weimin
The Rockefeller University
Wax Robert A.
LandOfFree
Chimeric proteins comprising liver enriched transcription factor does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chimeric proteins comprising liver enriched transcription factor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chimeric proteins comprising liver enriched transcription factor will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1905085