Chimeric proteins comprising a Vpr/Vpx virion incorporation doma

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Fusion protein or fusion polypeptide

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4241841, 4241851, 4241881, 530300, 530350, A61K 3900, A61K 3921, A61K 3800, C07K 100

Patent

active

058611614

ABSTRACT:
The present invention is directed toward chimeric human immunodeficiency virus type 1 and 2 (HIV-1 and -2) proteins that are capable of being incorporated into the virion when expressed in trans. These chimeric proteins consist of a first portion comprising an HIV-1 or HIV-2 Vpr/Vpx virion incorporation domain which includes the predicted N-terminal alpha helix. This domain is capable of interacting with the p6 domain of the Pr55.sup.gag. The second portion of the chimera comprises a sequence containing RNase activity, protease activity, or a domain capable of inhibiting virion morphogenesis and assembly. The disclosed invention provides a chimera that affects the structural organization or functional integrity of the mature virion by enzymatic disturbance or steric hindrance of the virion.

REFERENCES:
Kappes et al., 1993, "Intracellular transport and virion incorporation of vpx requires interaction with other virus type-specific components", Virol. 193:222-233.
Matsuda et al., 1993, Proc. Natl. Acad. Sci. USA 90:3544-3548.
Kim et al., 1992, AIDS Res. Hum. Retro. 8:1033-1038.
Feinberg et al., 1992, AIDS Res. Hum. Retro. 8:1013-1022.
Kondo E. et al., 1995, Journal of Virology 69:2759-2764.
Kappes J.C. et al., 1995, Keystone Symposium on Gene Therapy and Molecular Medicine, Journal of Cellular Biochemistry Supplement, p. 395.
Wu X. et al., 1994, Journal of Virology 68:6161-6169.
Horton R. et al., 1994, Virology 199:453-457.
Cohen et al., 1988, Nature, 334:532-534.
Lavallee et al., 1994, J. Virol., 68:1925-1934.
Levy et al., 1993, Cell, 72:541-550.
Lu et al., 1993, Journal of Virology, 67(1):6542-6550.
Myers et al., 1993, Human Retroviruses and AIDS 1993 AI-II Los Alamos National Laboratory, New Mexico, USA.
Paxton et al., 1993, Journal of Virology, 67(12):7229-7237.
Wagner et al., 1994, Virology, 200:162-175.
Wills, 1989, Nature, 340:323-324.
Yao et al., 1992, Journal of Virology, 66(8):5119-5126.
"IUPAC-IUB Commission on Biochemical Nomenclature." Biochemistry, 1972, 11:1726-1732.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Chimeric proteins comprising a Vpr/Vpx virion incorporation doma does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chimeric proteins comprising a Vpr/Vpx virion incorporation doma, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chimeric proteins comprising a Vpr/Vpx virion incorporation doma will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1245022

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.