Chimeric protein that has a human RHo Motif and deoxyribonucleas

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435199, 43525411, C12N 1553, C12N 115, C12N 922

Patent

active

053248309

ABSTRACT:
The present invention relates, in general, to a DNA segment. In particular, the present invention relates to a DNA segment coding for a polypeptide having an amino acid sequence corresponding to RhoNUC, a polypeptide having an amino acid sequence corresponding to RhoNUC, antibodies to RhoNUC, a recombinant DNA molecule, a cell containing the recombinant DNA molecule, and methods of producing and using the polypeptide and DNA segment.

REFERENCES:
Chow, T. Y. K., et al. (1988) Mol. Gen. Genet. 211, 41-48.
Chow, T. Y. K., et al, (1987) J. Biol. Chem. 262(36), 17659-17667.
Suggs, S. V. et al. (1981) Proc. Natl. Acad. Sci. USA 78(11), 6613-6617.
Esposito, et al.; Current Genetics 1986 10:425-433; "The REC46 gene of Saccharomyces cerevisiae controls mitotic chromosomal stability, recombination and sporulation: cell-type and life cycle stage-specific expression of the rec46-1 mutation".
Kouprina, et al.; Yeast vol. 4:257-269 (1988); "Genetic Control of Chromosome Stability in the Yeast Saccharomyces cerevisiae".
Fitzgerald-Hayes, Molly; Yeast; vol. 3: 187-200 (1987); "Yeast Centromeres".
Rosenberg, S. and Hastings, P., "The split-end model for homologous recombination at double-strand breaks and at Chi," Biochimie 73:385-397 (1987).
Fraser, et al., "Neurospora Endo-Exonuclease Is Immunochemically Related to the recC Gene Product of Escherichia coli," J. Bacteriology 172-507-510 (1990).
Kunz, B. and Haynes R., "Phenomenology and Genetic Control of Mitotic Recombination in Yeast," Ann. Rev. Genet. 15:57-89 (1981).
Koa, H. et al., "Endo-exonuclease of Aspergillus nidulans," Biochem. Cell Biol. 68:387-392 (1989).
Dake, et al., "Purification and Properties of the Major Nuclease from Mitochondria of Saccharomyces cerevisiae," J. Biol. Chem 263:7691-7702 (1988).
Chardin, P. et al., "Coding Sequence of human rho cDNAs clone 6 and clone 9," Nucleic Acids Research 16:2717 (1988).
Yeramian, P. et al., "Nucleotide sequence of human rho cDNA clone 12" Nucleic Acids Research 15:1869 (1987).
Madaule, P. et al., "Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae" Proc. Natl. Acad. Sci. USA 84:779-783 (1987).
Ramotar, D. et al., "Nuclear Endo-exonuclease of Neurospora crassa" J. Biol. Chem. 262:425-431 (1987).
Tomkinson, A. and Linn, S., "Purification and properties of a single strand-specific endonuclease from mouse cell mitochondria" Nucleic Acids Research 14:9579-9593 (1986).
Snyder, M. et al., "Rapid mapping of antigenic coding regions and constructing insertion mutations in yeast genes by mini-Tn10 transplason mutagenesis" Proc. Natl. Acad. Sci. USA 83:730-734 (1986).
Fraser, M. et al., "An immunochemical study of Neurospora nucleases" Biochem. Cell Biol. 64:106-116.
Madaulel, P. and Axel, R., "A novel ras-Related Gene Family," Cell. 41:31-40 (1985).
Devereux, J. et al., "A comprehensive st of sequence analysis programs for the V A X," Nucleic Acids Research 12:387-395 (1984).
Resnick, M. et al., "DNA Polymerases, Deoxyribonucleases, and recombination during meiosis in Saccharaomes cerevisiae" Mol. Cell Biol. 4:2811-2817 (1984).
Chaudhury A. and Smith G., "A new class of Escherichia coli recBC mutants: Implications for the role of RecBC enzyme in homologous recombination," Proc. Natl. Acad. Sci. USA 81:7850-7854 (1984).
Rothestein, R., "One-step gene disruption in yeast" Methods in Enzymology 101:202-221 (1983).
Chow, T. and Fraser, M., "Purification and properties of single strand DNA-binding endo-exonuclease of Neurospora crassa" J. Biol. Chem. 258:12010-12018 (1983).
Elder, T. et al., "RNA from the yeast transposable element tyl has both ends in the direct repeats a structure similar to retrovirus RNA" Proc. Natl. Acad. Sci. USA 80:2432-2436 (1983).
Young, A. and Davis, R., "Efficient isolation of genes by using antibody probes" Proc. Natl. Acad. Sci. USA 80:1194-1198 (1983).
Malone, R. and Esposito, R., "The RAD52 gene is required for homothallic interconversion of mating types and spontaneous mitotic recombination in yeast," Proc. Natl. Acad. Sci. USA 77:503-507 (1980).
Towbin, H. et al., "Electrophoretic transfer of proteins from polyacrylamide gels to mitrocellulose sheets: Procedure and some applications," Proc. Natl. Acad. Sci. USA 76:4350-4354 (1979).
Chow, T. and Fraser, M., "The major intracellular alkali deoxyribonuclease activities expressed in wild-type and Rec-like mutants of Neurospora crassa" Can. J. Biochem. 57:889-901 (1979).
Sanger F. and Coulson, A., "The use of thin acrylamide gels for DNA sequencing" MRS Lab. Mol. Biol. 87:107-110 (1978).
McMaster G. and Charmichael, G., "Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange" Proc. Natl. Acad. Sci. USA 74:4835-4838 (1977).
Berk, A. and Phillip, A., "Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids" Cell. 12:721-731 (1977).
Resnick, M. and Martin, P., "The repair of double-strand breaks in the Nuclear DNA of Saccharomyces cerevisiae and its genetic control" Molec. gen. Genet. 143:119-129 (1976).
Kohler, G. and Milstein C., "Continuous cultures of fused cells secreting antibody of predefined specifity" Nature 256:495-497 (1975).
Zhu, H. et al., "Determination of the carboxyl termini of the alpha and beta subunits of yeast K1 killer toxin" J. Biol. Chem. 262:10728-10732 (1987).
Ho, K. and Mortimer, R., "Induction of dominant lethality by x-rays in a radiosensitive strain of yeast," Mutation Research 20:45-51 (1973).
Hollowman, W. and Holliday, R., "Studies on a nuclease from Ustilago maydis" J. Biol. Chem. 23:8107-8113 (1973).
Fraser, M. et al., "Nucleases and their control in wild-type and nuh mutants for neurospora" Dept. Biochem. Biol. McGill University Chapter 5:63-74 (1979).
Young, R. and Davis, R., "Yeast RNA polymerase II genes; Isolation with antibody probes," Science 222:778-782 (1983).
Chow, T. et al., "Repair and recombination defective mei-41 mutants of drosophila lack a Dnase which is related to nucleases from fungi" J. Cell. Biochem. 10B:211 (1986), Abst. No. 1245.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Chimeric protein that has a human RHo Motif and deoxyribonucleas does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chimeric protein that has a human RHo Motif and deoxyribonucleas, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chimeric protein that has a human RHo Motif and deoxyribonucleas will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2378179

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.