Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1992-07-14
1996-02-06
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435193, C12N 922
Patent
active
054895243
ABSTRACT:
The present invention relates, in general, to endo-exonucleases. In particular, the present invention relates to DNA segments encoding for a polypeptide having an amino acid sequence corresponding to mammalian endo-exonuclease, a polypeptide having an amino acid sequence corresponding to mammalian endo-exonuclease, antibodies to mammalian endo-exonuclease, a recombinant DNA molecule, mutant cells substantially lacking endo-exonuclease activity, a cell containing mammalian endo-exonuclease, and methods of producing and using the polypeptide, DNA segment and mutants.
REFERENCES:
Barondess, J. T., et al., (1990) Nature 346, 871-874.
Sanger, F., et al, (1982) J. Mol. Biol. 162, 729-773.
Chow, T. & Resnick, M., "Purification and characterization of an endoexonuclease from Saccharomyces cerevisiae that is influenced by the RAD52 gene," Journal of Biological Chemistry, vol. 262, No. 36, 25 Dec. 1987, Baltimore, Maryland, pp. 17659-17667.
Moran, L. et al., "Nucleotide sequence of the phage lambda gt11 SacI-KpnI lacZ region," Gene, vol. 93, 1990, Amsterdam, Netherlands, pp. 163-164.
Resnick, M. et al., Database WPI, Section Ch, Week, 9149, Derwent Publications Ltd., London, GB, Class B04, AN 91-361692 and US-Published-Patent-Application 7674801. (1991).
Chien, C. T. et al., "The two-hybrid system: a method to identify and clone genes proteins that interact with a protein of interest," Proc. Nat. Acad. of Sciences, U.S.A., vol. 88, No. 1991, Washington, US, pp. 9578-9582.
Chow, T. et al., Database WPI, Section Ch, Week 9318, Derwent Publications Ltd., London, GB, Class B04, AN 93-152048, and US-Published-Patent-Application 7914284. (1992).
Couture C. & T. Chow et al., "Purification and characterization of mammalian endo-exonuclease," Nucleic Acids Research, vol. 20, No. 16, 25 Aug. 1992, Arlington, Va., pp. 4355-4361.
Rosenberg, S. and Hastings, P., "The split-end model for homologous recombination at double-strand breaks and at Chi," Biochimie 73:385-397 (1987).
Fraser, et al., "Neurospora Endo-Exonuclease Is Immunochemically Related to the recC Gene Product of Escherichia coli," J. Bacteriology 172:507-510 (1990).
Kunz, B. and Haynes, R., "Phenomenology and Genetic Control of Mitotic Recombination in Yeast," Ann. Rev. Genet. 15:57-89 (1981).
Koa, H. et al., "Endo-exonuclease of Aspergillus nidulans," Biochem. Cell Biol. 68:387-392 (1989).
Dake, et al., "Purification and Properties of the Major Nuclease from Mitochondria of Saccharomyces cerevisiae," J. Biol. Chem. 263:7691-7702 (1988).
Chardin, P. et al., "Coding Sequence of human rho cDNAs clone 6 and clone 9," Nucleic Acids Research 16:2717 (1988).
Chow, T. and Resnick M., "An endo-exonuclease activity of yeast that requires a functional RAD52 gene," Mol. Gen. Genet 211:41-48 (1988).
Yeramian, P. et al., "Nucleotide sequence of human rho cDNA clone 12" Nucleic Acids Research 15:1869 (1987).
Madaule, P. et al., "characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae" Proc. Natl. Acad. Sci. USA 84:779-783 (1987).
Ramotar, D. et al., "Nuclear Endo-exonuclease of Neurospora crassa" J. Biol. Chem. 262:425-431 (1987).
Tomkinson, A. and Linn, S., "Purification and properties of a single strand-specific endonuclease from mouse cell mitochondria" Nucleic Acids Research 14:9579-9593 (1986).
Snyder, M. et al., "Rapid mapping of antigenic coding regions and constructing insertion mutations in yeast genes by mini-Tn10 transplason mutagenesis" Proc. Natl. Acad. Sci. USA 83:730-734 (1986).
Fraser, M. et al., "An immunochemical study of Neurospora nucleases" Biochem. Cell Biol. 64:106-116.
Madaulel, P. and Axel, R., "A novel ras-Related Gene Family, " Cell. 41:31-40 (1985).
Devereux, J. et al., "A comprehensive st of sequence analysis program for the V A X," Nucleic Acids Research 12:387-395 (1984).
Resnick, M.. et al., "DNA Polymerases, Deoxyribonucleases, and recombination during meiosis in Saccharaomes cerevisiae" Mol. Cell Biol. 4:2811-2817 (1984).
Chaudhury A. and Smith G., "A new class of Escherichia coli recBC mutants: Implications for the role of RecBC enzyme in homologous recombination," Proc. Natl. Acad. Sci. USA 81:7850-7854 (1984).
Rothestein, R., "One-step gene disruption in yeast" Methods in Enzymology 101:202-221 (1983).
Chow, T. and Fraser, M., "Purification and properties of single strand DNA-binding endo-exonuclease of Neurospora crassa" J. Biol. Chem. 258:12010-12018 (1983).
Elder, T. et al., "RNA from the yeast transposable element tyl has both ends in the direct repeats a structure similar to retrovirus RNA" Proc. Natl. Acad. Sci. USA 80:2432-2436 (1983).
Young, A. and Davis, R., "Efficient isolation of genes by using antibody probes" Proc. Natl. Acad. Sci. USA 80:1194-1198 (1983).
Malone, R. and Esposito, R., "RAD52 gene is required for homothallic interconversion of mating types and spontaneous mitotic recombination in yeast," Proc. Natl. Acad. Sci. USA. 77:503-507 (1980).
Towbin, H. et al., "Electrophoretic transfer of proteins from polyacrylamide gels to mitrocellulose sheets: Procedure and some applications," Proc. Natl. Acad. Sci. USA 76:4350-4354 (1979).
Chow, T. and Fraser, M., "The major intracellular alkali deoxyribonuclease activities expressed in wild-type and Rec-like mutants of Neurospora crassa" Can. J. Biochem. 57: 889-901 (1979).
Sanger, F. and Coulson, A., "The use of thin acrylamide for DNA sequencing" MRS Lab. Mol. Biol. 87:107-110 (1978).
McMaster G. and Charmichael, G., "Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange" Proc. Natl. Acad. Sci. USA 74:4835-4838 (1977).
Berk, A. an Phillip, A., "Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids" Cell. 12:721-731 (1977).
Resnick,M. and Martin, P., "The repair of double-strand breaks in the Nuclear DNA of Saccharomyces cerevisiae and its genetic control" Molec. gen. Genet. 143:119-129 (1976).
Kohler, G. and Milstein C., "Continuous cultures of fused cells secreting antibody of predefined specifity" Nature 256:495-497 (1975).
Zhu, H. et al., "Determination of the carboxyl termini of the alpha and beta subunits of yeast K1 killer toxin" J. Biol. Chem. 262:10728-10732 (1987).
Ho, K. and Mortimer, R., "Induction of dominant lethality by x-rays in a radiosensitive strain of yeast," Mutation Research 20:45-51 (1973).
Hollowman, W. and Holliday, R., "Studies on a nuclease from ustilago maydis" J. Biol. Chem. 23:8107-8113 (1973).
Fraser, M. et al., "Nucleases and their control in wild-type and nuh mutants for neurospora" Dept. Biochem. Biol. McGill University Chapter 5:63-74 (1979).
Young, R. and Davis, R., "Yeast RNA polymerase II genes; Isolation with antibody probes," Science 222:778-782 (1983).
Chow, T. et al., "Repair and recombination defective mei-41 mutants of drosophila lack a Dnase which is related to nucleases from fungi" J. Cell. Biochem. 10B:211 (1986).
Esposito, et al.; Current Genetics 1986 10:425-433; "The REC46 gene of Saccharomyces cerevisiae controls mitotic chromosomal stability, recombination and sporulation: cell-type and life cycle stage-specific expression of the rec46-1 mutation".
Kouprina, et al.; Yeast vol. 4:257-269 (1988); "Genetic Control of Chromosome Stability in the Yeast Saccharomyces cerevisiae".
Fitzgerald-Hayes, Molly; Yeast; vol. 3: 187-200 (1987) "Yeast Centromeres".
Chow and Resnick; Cellular Response to DNA Damage, pp. 447-455; 1983 "The Identification of A Deoxyribonuclease Controlled by the RAD52 Gene of Saccharomyces cerevisiae".
Hibino, et al.; Biochimica et Biophysica Acta, 1088 (1991) 305-307; BBA Report; "A Nuclease from Rat-Liver Nuclei with Endo- and Exonucleolytic Activity".
Chien, et al.; Proc. Natl. Acad. Sci. USA; vol. 88; pp. 9578-9582; Nov. 1991; Biochemistry; "The Two-Hybrid System: A Method to Identify and Clone Genes For Proteins that Interact with a Protein of Interest".
Sander, et al.; 1991 Oxford University Press: Nucleic Acids Research, vol. 19, No. 16, pp. 4523-4529; "Cloning and Characterization of Rrpl, The Gene Encoding Drosophila Str
Chow Terry
Perkins Edward L.
Resnick Michael A.
Patterson Jr. Charles L.
The United States of America as represented by the Department of
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