Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2005-07-12
2005-07-12
Ungar, Susan (Department: 1646)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C530S350000, C514S04400A
Reexamination Certificate
active
06916917
ABSTRACT:
The present invention relates to a chimeric pro-caspase, which contains a pro-caspase domain and an oligomerizing domain. The invention also relates to an antibody that reacts specifically with a chimeric pro-caspase. In addition, the invention further relates to a polynucleotide encoding a chimeric pro-caspase, and to nucleotide sequences, which can hybridize specifically with a polynucleotide encoding a chimeric pro-caspase. The present invention also relates to a method of inducing apoptosis in a cell by providing a chimeric pro-caspase in the cell, wherein the chimeric pro-caspase includes a pro-caspase domain and an oligomerizing domain, whereby the chimeric pro-caspase forms an oligomer in the cell, thereby activating caspase activity of the chimeric pro-caspase and inducing apoptosis in the cell. The present invention further relates to a method of reducing the severity of a pathologic condition in a subject, by providing cells of the subject that are involved in the pathologic condition with a chimeric pro-caspase comprising a pro-caspase domain and an oligomerizing domain, whereby the chimeric pro-caspase forms an oligomer in the cells, thereby activating caspase activity of the chimeric pro-caspase, inducing apoptosis in the cells, and reducing the severity of the pathologic condition in the subject.
REFERENCES:
patent: 4889806 (1989-12-01), Olson et al.
patent: 5837837 (1998-11-01), Hunter et al.
Miller FASEB .j, 9:190-199, 1995.
Deonarian. Expert. Opin. Ther. Pat. 8. 53-69, 1998.
Verma .Nature, 389: 239-242, 1997.
Crystal Science 270 : 404-410, 1995.
Meergans, T. Brocheru J. 349(1):135-140, 2000.
Muzio. J. Biol. Chem, 273(5): 2926-2930, 1998.
Masako, U. Intl. J. Oncol. 20(3):617-22, 2002.
Browes, C. FEBS letters, 497(1):20-5, 2001.
Bider, M D J.B.C. 271:31996-2001, 1996.
Grika. Science, 278: 1041-1042, 1997.
Jain Sci Amer 271:58-65, 1994.
Curti. Crit. Rev. Oncol./Hematol. 14:29-39, 1993.
Hartwell. Science 278:1064-1068, 1997.
Yang, X. Mol. Cell. 1:319-325, 1998.
Sambrook, J. Mol. Cloning, Cold Spring Harbor Press, Cold Spring Harbor, p. 16.3-16.4, 16.26-16.27, 1989.
Boehringer Mannheim, Biochemicals Catalog, p. 93, 1994.
Chang, D. W., et al., “Oligomerization Is a General Mechanism for the Activation of Apoptosis Initiator and Inflammatory Procapases,”The Journal of Biological Chemistry, vol. 278, No. 19, 2003, pp. 16466-16469.
Hu, Y., et al., “WD-40 Repeat Region Regulates Apaf-1 Self-association and Procaspase-9 Activation,”The Journal of Biological Chemistry, vol. 273, No. 50, Dec. 11, 1988, pp. 33489-33494.
Martin, D.A. et al., “Membrane Oligomerization and Cleavage Activates the Caspase-8 (FLICE/MACHα1) Death Signal,”The Journal of Biological Chemistry, vol. 273, No. 8, Feb. 20, 1998, pp. 4345-4349.
Srinivasula, S.M., et al., “Autoactivation of Procaspase-9 by Apaf-1-Mediated Oligolerization,”Molecular Cell, vol. 1, Jun., 1998, pp. 949-957.
Cohen, G.M., “Caspases: the executioners of apoptosis,”Biochem. J. 326:1-16 (1997).
Schwarze, et al, “In Vivo Protein Transduction: Delivery of a Biologically Active Protein into the Mouse,”Science285:1569-1572(1999).
Thornberry and Lazebnik, “Caspases: Enemies Within,”Science281:1312-1316 (1998).
Vocero-Akbani, et al., “Killing HIV-infected cells by transduction with an HIV protease-activated caspase-3 protein,”Nature Medicine3(1):29-33(1999).
Yang, et al., “Autoproteolytic Activation of Pro-Caspases by Oligomerization,”Molecular Cell1:319-325 (1998).
Yang, et al., “Essential Role of CED-4 Oligomerization in CED-3 Activation and Apoptosis,”Science281:1355-1357 (1998).
Baltimore David
Chang Howard Y.
Yang Xiaolu
California Institute of Technology
Davis Minh-Tam
Gray Cary Ware & Freidenrich LLP
Ungar Susan
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