Chemistry: molecular biology and microbiology – Vector – per se
Patent
1987-11-18
1989-10-24
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Vector, per se
435170, 4351723, 43525231, 43525233, 536 27, 935 29, 935 56, C12N 700, C12N 1500, C07H 1512
Patent
active
048762024
ABSTRACT:
A variety of chimeric plasmids each of which comprises (a) a tetracycline resistance gene (Tc) deriving from pAM.alpha.1, one of the plasmids retained by Streptococcus faecalis, (b) an ampicillin resistance gene (Amp) deriving from pACYC177, one of the vectors applicable to Escherichia coli and (c) either or both of origins, an origin (OripAM.alpha.1) deriving from the plasmid pAM.alpha.1 and an origin (Ori177) deriving from the vector pACYC177, the tetracycline resistance gene existing on each of which has the unique cleavage site for the restriction enzyme BalI on the entire DNA of the same plasmid and the ampicillin resistance gene existing on each of which has the unique cleavage sites for respective restriction enzymes BglI and PstI on the entire DNA on the same plasmid.
There is also provided a chimeric plasmid vector containing (a) a tetracycline resistance gene region (Tc) derived from the plasmid pAM.alpha.1 of Streptococcus faecalis DS5 (ATCC14508), (b) an ampicillin resistance gene region (Amp) derived from the vector pACYC177 of E. coli, (c) a first DNA replication origin (OripAM.alpha.1) derived from the plasmid pAM.alpha.1, (d) a second DNA replication origin (Ori177) derived from the vector pACYC177, and (e) a polylinker region having recognition and cleavage sites for the restriction enzymes EcoRI at one terminal and HindIII at the other terminal of the polylinker DNA sequence.
REFERENCES:
patent: 4595660 (1986-06-01), Ostroff et al.
Gray et al., 1981, J. Bacterial, 145:422-428.
Chang et al., 1978, J. Bacteriol, 134:1141-1155.
Clewell et al., 1975, PNAS, 72:1720-1724.
Maniatis et al., 1982, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory.
Burdett et al., "Multiple Tetracycline Resistance Determinants in Streptococcus", Microbiology, 1982.
Bal et al., Acta Microbiologica Polonica, 32 (34), 217-225, 1982.
Ishiwa Hiromi
Mutai Masahiko
Shibahara Harue
Tsuchida Nobuo
Kabushiki Kaisha Yakul't Honsha
Peet Richard C.
Weimar Elizabeth C.
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