Chimeric plant genes based on upstream regulatory elements of he

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800DIG14, 800DIG17, 800DIG26, 800DIG27, 800DIG43, 800DIG56, 4353201, 4351723, 4352404, 536 236, 536 241, C12N 1529, C12N 1582, C12N 504, A01H 500

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058248650

ABSTRACT:
Helianthinin is an 11S seed storage protein of sunflower embryos. The present invention is directed to the 5' regulatory regions of helianthinin genes. More particularly, the present invention is directed to specific cis-regulatory elements of this regulatory region which direct abscisic acid-responsive gene expression. The present invention provides chimeric genes comprising the cis-regulatory elements linked to a coding sequence from a heterologous gene to control expression of these genes. The chimeric genes provided by the instant invention are useful in conferring herbicide resistance and improved seed lipid quality to transgenic plants.

REFERENCES:
Bogue et al. (1990) "Developmentally regulated expression of a sunflower 11S seed protein gene in transgenic tobacco" Molecular and General Genetics 222:49-57.
Thomas et al. (1991) "ABA regulation of gene expression in embryos and mature plants" In: Abscisic Acid: Psychology and Biochemistry, (Ed. by Davis and Jones) 125-136.
Thomas et al. (1991) "ABA regulation of two distinct plant embryo genes" J. Cell Biochem. (Suppl), 18A:126.
Thomas et al. (1991) "ABA regulation of gene expression in embryos and mature plants" J. Exp. Bot., 42:10.
Bustos et al. (1989) Plant Cell I, 839-853.
Chen et al. (1988) Embo Journal 7, 297-302.
Colot et al. (1987) Embo Journal 6, 3559-3564.
Goldberg et al. (1989) Cell 56, 149-160.
Jordano et al. (1989) Plant Cell I, 855-866.
Marris et al. (1988) Plant Molecular Biology 10, 359-366.
Schernthaner et al. (1988) Embo Journal 7, 1249-1255.
Vonder Harr et al. (1988) Gene 74, 433-443.
Wada et al., Nature, 347: 200-203 (1990).

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