Chimeric nitrilase-encoding gene for herbicidal resistance

Details Chimeric nitrilase-encoding gene for herbicidal resistance Chimeric nitrilase-encoding gene for herbicidal resistance

1995-01-13

1996-09-24

Chereskin, Che S.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

536 232, 4353201, 4352404, 800205, C12N 510, C12N 1555, C12N 1531

Patent

active

055590241

ABSTRACT:
A Chimeric gene which is usable for endowing plants with resistance to a herbicide based on 3,5-dihalo-4-hydroxy-benzonitrile, comprising at least one gene coding for resistance to this herbicide, a foreign promoter and, optionally, a polyadenylation signal region, wherein the promoter originates from a gene which is naturally expressed in plant cells and is chosen from the group comprising the promoter of the 35 S RNA for cauliflower mosaic virus (CaMV 35S) and the promoter of the small subunit (SSU) of sunflower (Helianthus annuus) ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO).

REFERENCES:
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B. Leroux, et al., "A new selectable marker to study gene expression in plants", Journal of Cellular Biochemistry, Supplement 12C (1988), UCLA Symopsia on Molecular & Cellular Biology, Abstracts, No. L513, 17th Annual Meetings, Feb. 28-Apr. 10, 1988, p. 201.
G. Waksman, et al, "Nucleotide sequence of a gene encoding sunflower ribulose-1,5-bisphosphate crboxylase/oxygenase small subunit (rbcs)", Nucleic Acids Research, vol. 15, No. 17 (1987), p. 7181.
M. Lebrun, et al., "Nucelotide sequence of a gene encoding corn ribulose-1,5-bisphosphate carboxylase/oxygenase small subnit (rbcs)", Nucleic Acids Research, vol. 15, No. 10 (1987), p. 4360.
R. A. Jefferson, et al., "GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants", The EMBO Journal, vol. 6, No. 13 (1987), pp. 3901-3907.
R. Topfer, et al., "A set of plant expression vectors for transcriptional and translational fusions", Nucleic Acids Research, vol. 15, No. 14 (Jul. 24, 1987), p. 5890.
C. L. Schardl, et al., "Design and contruction of a versatile system for the expression of foreign genes in plants", Gene, vol. 61, No. 1 (1987), pp. 1-11.

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