Chimeric nicotinic receptor subunits

Details Chimeric nicotinic receptor subunits Chimeric nicotinic receptor subunits

2005-01-18

2009-06-23

Saoud, Christine J (Department: 1647)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S069100, C435S252300, C435S320100, C530S350000, C536S023500

Reexamination Certificate

active

07550293

ABSTRACT:
A chimeric nAChR receptor subunit polypeptide having a substitution of at least about 15% of the native amino acid sequence of the subunit in the area of the C-terminal cytoplasmic domain is provided, as well as polynucleotides encoding the polypeptide. Vectors, host cells, and related methods for evaluating compounds are also provided.

REFERENCES:
patent: 4631211 (1986-12-01), Houghten
patent: 4873191 (1989-10-01), Wagner
patent: 5187166 (1993-02-01), Kikuchi
patent: 5583140 (1996-12-01), Bencherif
patent: 5597919 (1997-01-01), Dull
patent: 5641670 (1997-06-01), Treco
patent: 5672601 (1997-09-01), Cignarella
patent: 5852041 (1998-12-01), Cosford
patent: 6638925 (2003-10-01), Czollner
patent: 2045869 (1991-12-01), None
patent: 0 297 858 (1989-01-01), None
patent: 2 295 387 (1996-05-01), None
patent: WO 87/01130 (1987-02-01), None
patent: WO 93/13423 (1993-07-01), None
patent: WO 94/08992 (1994-04-01), None
patent: WO 94/12650 (1994-06-01), None
patent: WO 96/29411 (1996-09-01), None
patent: WO 96/31475 (1996-10-01), None
patent: WO 96/40682 (1996-12-01), None
patent: WO 99/21834 (1999-05-01), None
Wells, J.A. (1990). Additivity of mutational effects in proteins. Biochemistry. 29(37):8509-8517.
Ngo et al. (1994). Computational complexity, protein structure prediction, and the Levinthal paradox. In Merz and Le Grand (Eds.) The Protein Folding Problem and Tertiary Structure Prediction. Birkhauser:Boston, pp. 491-495.
Skolnick et al. (2000). From genes to protein structure and function: novel applications of computational approaches in the genomic era. Trends in Biotechnology. 18:34-39.
Wang et al. (1999). Rapid analysis of gene expression (RAGE) facilitates universal expression profiling. Nucleic Acds Research. 27(23):4609-4618.
Kaufman et al. (1999). Transgenic analysis of a 100-kb human B-globulin cluster-containing DNA fragment propagated as a bacterial artificial chromosome. Blood. 94(9):3178-3184.
Arneric, S., et al., “Preclinical Pharmacology of ABT-418: A Prototypical Cholinergic Channel Activator for the Potential Treatment of Alzheimer's Disease,”CNS Drug Rev., 1(1): 1-26 (1995).
Arneric, S., et al., “Cholinergic channel modulators as a novel therapeutic strategy for Alzheimer's disease,”Exp. Opin. Invest. Drugs, 5(1): 79-100 (1996).
Bannon, A.W., et al., “Broad-Spectrum, Non-Opioid Analgesic Activity by Selective Modulation of Neuronal Nicotinic Acetylcholine Receptors,”Science, 279: 77-80 (1998).
Bencherif, M., et al., “RJR-2403: A Nicotinic Agonist with CNS Selectivity I: In Vitro Characterization,”J. Pharmacol. Exper. Therapeutics, 279(3): 1413-1421 (1996).
Bencherif, M, and R.J. Lukas, “Cytochalasin Modulation of Nicotinic Cholinergic Receptor Expression and Muscarinic Receptor Function in Human TE671/RD Cells: A Possible Functional Role of the Cytoskeleton,”Journal of Neurochemistry, 61: 852-864 (1993).
Bennett, D., et al., “Kinetic Characterization of the Interaction of Biotinylated Human Interleukin 5 with an Fc Chimera of its Receptor α Subunit and Development of an ELISA Screening Assay using Real-Time Interaction Biosensor Analysis,”J. Mol. Recog., 8: 52-58 (1995).
Boulter, J., et al., “Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor α-Subunit,”Nature, 319(6052): 368-374 (1986).
Brioni, J.D., et al., “The Pharmacology of (−)-Nicotine and Novel Cholinergic Channel Modulators,”Adv. Pharmacol., 37: 153-215 (1997).
Brutlag, D.L., et al., “Improved sensitivity of biological sequence database searches,”Comp. Ap. Biosc.6(3): 237-245 (1990).
Buckle, V.J., et al., “Chromosomal Localization of GABAAReceptor Subunit Genes: Relationship to Human Genetic Disease,”Neuron., 3(5): 647-654 (1989).
Campbell, K.H.S., et al., “Sheep cloned by nuclear transfer from a cultured cell line,”Nature, 380: 64-66 (1996).
Carver, A.S., et al., “Transgenic Livestock as Bioreactors: Stable Expression of Human Alpha-1-Antitrypsin by a Flock of Sheep,”Biotechnology, 11: 1263-1270 (1993).
Campos-Caro, A., et al., “A single residue in the M2-M3 loop is a major determinant of coupling between binding and gating in neuronal nicotinic receptors,”Pro. Natl. Acad. Sci., 93(12): 6118-6123 (1996).
Chiari, A., et al., “Sex Differences in Cholinergic Analgesia I: A Supplemental Nicotinic Mechanism in Normal Females,”Anesthesiology, 91(5): 1447-1454 (1999).
Cleland, J.L., et al., “The Development of Stable Protein Formulations: A Close Look at Protein Aggregation, Deamidation, and Oxidation,”Crit. Rev. Therapeutic Drug Carrier Systems, 10(4): 307-377 (1993).
Clementi, F., et al., “Pharmacological Characterization of Cholinergic Receptors in a Human Neuroblastoma Cell Line,”J. Neurochem., 47(1): 291-297 (1986).
Cleveland, D.W., et al., “Number and Evolutionary Conservation of a α- and β- Tubulin and Cytoplasmic β- and γ- Actin Genes Using Specific Cloned cDNA Probes,”Cell, 20: 95-105 (1980).
Colledge M. and S.C. Froehner, “Tyrosine Phosphorylation of Nicotinic Acetylcholine Receptor Mediates Grb2 Binding,”J. Neurosci., 17(13): 5038-5045 (1997).
Cooper, S.T., and N.S. Millar, “Host Cell-Specific Folding of the Neuronal Nicotinic Receptor α8 Subunit,”J. Neurochem., 70: 2585-2593 (1998).
Cooper, S.T., et al., “Up-regulation of Cell-surface α4β2 Neuronal Nicotinic Receptors by Lower Temperature and Express of Chimeric Subunits,”The Journal of Biological Chemistry, 274(38): 27145-27152 (1999).
Corringer P-J, et al., “Nicotinic Receptors at the Amino Acid Level,”Annu. Rev. Pharmacol. Toxicol., 40: 431-458 (2000).
Cunningham, B.C., and J.A. Wells, “High-Resolution Epitope Mapping of hGH-Receptor Interactions by Alanine-Scanning Mutagenesis,”Science, 244: 1081-1085 (1989).
Dajas-Bailador, F.A., et al., “Nicotine activates the extracellular signal-regulated kinase ½ via the α7 nicotinic acetylcholine receptor and protein kinase A, in SH-SY5Y cells and hippocampal neurons,”J. Neurochem., 80: 520-530 (2002).
Damaj, M.I., et al., “Antinociceptive and Pharmacological Effects of Metanicotine, a Selective Nicotinic Agonist,”J. Pharmacol. Exp. Ther., 291(1): 390-398 (1999).
Dascal, D., “The Use ofXenopusOocytes for the Study of Ion Channels,”Crit. Rev. Biochem., 22(4): 317-387(1987).
Decina, P., et al. “Cigarette Smoking and Neuroleptic-Induced Parkinsonism,”Biol. Psychiatry, 28: 502-508 (1990).
Deneris, E.S., et al., “Pharmacological and functional diversity of neuronal nicotinic acetylcholine receptors,”TIPS, 121: 34-40 (1991).
Devillers-Thiery, A., et al., “Functional Architecture of the Nicotinic Acetylcholine Receptor: A Prototype of Ligand-gated Ion Channels,”J. Membrane Biol., 136: 97-112 (1993).
De Vos, A., et al., “Human Growth Hormone and Extracellular Domain of Its Receptor: Crystal Structure of the Complex,”Science, 255: 306-312 (1992).
Eaton, J.B., et al., “Characterization of Human α4β2—Nicotinic Acetylcholine Receptors Stably and Heterologously Expressed in Native Nicotinic Receptor-Null SH-EP1 Human Epithelial Cells,”Mol. Pharmacol., 64(6): 1283-1294 (2003).
Eisele, J-L., et al., “Chimaeric nicotinic-serotonergic receptor combines distinct ligand binding and channel specificities,”Nature, 366: 479-483 (1993).
Fenster, C.P., et al., “Regulation of α4β2 Nicotinic Receptor Desensitations by Calcium and Protein Kinase C,”C. Mol. Pharmacol., 55: 432-443 (1999).
Fenster, C.P., et al., “Upregulation of Surface α4β2 Nicotinic Receptors Is Initiated by Receptor Desensitization after Chronic Exposure to Nicotine,”The Journal of Neuroscience, 19(12): 4804-4814 (1999).
Gentz, R., et al., “Bioassay for tr

Affiliated with

Also associated with

Rating

Chimeric nicotinic receptor subunits does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chimeric nicotinic receptor subunits, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chimeric nicotinic receptor subunits will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4061681