Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-06-17
1998-03-24
Ketter, James
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
536 231, C12N 1500, C07H 2100
Patent
active
057311811
ABSTRACT:
The application concerns the design and use of small, duplex oligonucleotides and oligomers of nucleotide analogs, termed Chimeric Mutational Vectors (CMV) to effect mutation in a target gene of a eukaryotic cell by homologous recombination between the CMV and the target. The CMV comprises ribonucleotides and deoxyribonucleotides and nucleotide analogs of each (generically "nucleobases"). The application discloses that CMV contain at least two segments of at least 3 ribo-type nucleobases paired to deoxyribo-type nucleobases, which regions flank the region of the CMV that corresponds to the mutation to be introduced into the target gene. The ribo-type nucleobases should preferably be nuclease resistant i.e., other than naturally occurring ribonucleotides. The uses of CMV include gene therapy of genetic diseases and construction of transgenic plants and animals.
REFERENCES:
patent: 4503151 (1985-03-01), Paddock
patent: 4882279 (1989-11-01), Cregg
patent: 5013830 (1991-05-01), Ohtsuka et al.
patent: 5466786 (1995-11-01), Buhr et al.
patent: 5565350 (1996-10-01), Kmiec
patent: 5589369 (1996-12-01), Seidman et al.
Nielsen et al. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science vol. 254 1497-1500, 1991.
Lehninger Biochemistry, Second Edition Worth Publishers, Inc. 309-333 1975.
Shimizu et al., "Oligo(2'-O-methyl)ribonucleotides--Effective probes for duplex DNA", FEBS Letters 302:2,155-158 (1992).
Monia et al., "Selective Inhibition of Mutant Ha-ras mRNA Expression by Antisense Oligonucleotides", J Biol Chem 267:28,19954-19962 (1992).
Roberts, R.W., & Crothers, D.M., "Stability and Properties of Double and Triple Helices: Dramatic Effects of RNA or DNA Backbone Compsition", Science 258:5087,1463-1466 (1992).
Monia et al., "Evaluation of 2'-Modified Oligonucleotides Containing 2'-Deoxy Gaps as Antisense Inhibitors of Gene Expression", J Biol Chem 268:19,14514-14522 (1993).
Lesnik et al., "Oligodeoxynucleotides Containing 2'-O-Modified Adenosine: Synthesis and Effect on Stability of DNA:RNA Duplexes", Biochemistry 32:7832-7838 (1993).
Shimizu et al., "Effects of 5-Methyl Substition in 2'-O-Methyloligo-(Pyrimidine) Nucleotides on Triple-Helix Formation", Bio & Med Chem Ltrs 4:8,1029-1032 (1994).
Kmiec et al., "The REC2 Gene Encodes the Homologous Pairing Protein of Ustilago Maydis", Mol. and Cell. Biol. 14:7163 (1994).
Yoon et al., "Targeted Gene Correction of Episomal DNA in Mammalian Cells Mediated by a Chimeric RNA-DNA Oligonucleotide", Proc. Natl. Acad. Sci. USA 93:2071 (Mar. 1996).
Hosono, K., et al., "Properties and Anti-HIV Activity of Hairpin Antisense Oligonucleotides Containing 2'-Methoxynucleosides with Base-Pairing in the Stem Region at the 3'-end", Antiviral Chemistry & Chemotherapy 7:86-93 (Mar. 1996).
Brusca John S.
Hansburg Daniel
Ketter James
Thomas Jefferson University
LandOfFree
Chimeric mutational vectors having non-natural nucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chimeric mutational vectors having non-natural nucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chimeric mutational vectors having non-natural nucleotides will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2288050