Chimeric mouse for human and mouse immune systems

Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing – Testing efficacy or toxicity of a compound or composition

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800 8, 800 9, 800 11, 424 91, C12N 510, A61K 4900

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active

060541164

ABSTRACT:
This invention is directed to a chimeric mouse capable of mounting murine cellular and humoral immune response, where the chimeric mouse is tolerant of human tissue implanted therein. The chimeric mouse of this invention is capable of developing murine T cells and producing murine IgG antibodies, which T cells and antibodies are tolerant of the human tissue implanted in the mouse. This allows for the challenge of the vaccinated mouse with human-specific pathogens and determining the capacity of the vaccine to protect the cells in the implanted tissue from infection. This invention is also directed to a method for the development of the chimeric mouse, as well as to the use of the chimeric mouse for the screening of vaccines for human-specific pathogens.

REFERENCES:
"Development of Multiple Organ-Localized Autoimmune Diseases in Nude Mice After Reconstitution of T Cell Function by Rat Fetal Thymus Graft", by Taguchi et al., in J. Exp. Med., vol. 164, pp. 62-71, (Jul., 1986).
"Long-Term Human Hematopoiesis in the SCID-hu Mouse", by Namikawa et al., in J. Exp. Med., vol. 172, pp. 1055-1063 (Oct., 1990).
"The SCID-hu Mouse: Murine Model for the Analysis of Human Hematolymphoid Differentiation and Function", by McCune et al., in Science, vol. 241, pp. 1632-1639 (Sep. 23, 1988).
"Transfer of a Functional Human Immune System to Mice with Severe Combined Immunodeficiency", by Mosier et al., in Nature, vol. 335, pp. 256-259 (Sep. 1988).

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