Chimeric kanamycin resistance gene

Details Chimeric kanamycin resistance gene Chimeric kanamycin resistance gene

1996-05-06

1998-12-22

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4351723, 4352523, 4353201, 514 44, 536 231, 536 232, 536 236, 536 237, C12P 1934, C07H 2104, C12N 1554, C12N 1563

Patent

active

058518045

ABSTRACT:
Chimeric kanamycin resistance genes are disclosed. The chimeric genes comprise a nucleotide sequence that encodes ANT(4')-IA enzyme operably linked to a heterologous promoter and a heterologous termination sequence. Plasmids that comprise the chimeric kanamycin resistance gene are disclosed. Bacterial cells that comprise the chimeric gene on a plasmid or integrated into the bacterial genome are disclosed. Methods of producing plasmids are disclosed. Pharmaceutical compositions comprising plasmids that include the chimeric genes are disclosed. Methods of enhancing growth of a bacterial cells are disclosed. Plasmid which comprise the chimeric kanamycin resistance gene and the sequences from herpes simplex virus gene HSVgD.sub.2 or human immunodeficiency virus are disclosed.

REFERENCES:
patent: 4727028 (1988-02-01), Santerre et al.
patent: 4945050 (1990-07-01), Sanford et al.
patent: 5036006 (1991-07-01), Sanford et al.
patent: 5593972 (1997-01-01), Weiner et al.
PCT International Search Report dated Aug. 21, 1997, 1 page.
Deng et al., "Simultaneous randomiztion of antibody CDRs by a synthetic ligase chain reaction strategy", Nucl. Acids Res., 1993, 21(18), 4418-4419.
Pachuk et al., "Chain reaction cloning: A novel cloning strategy", FASEB J., 1996, 10(6), A1127, abstract No. 740.
Rouwendal et al., "Simultaneous mutagenesis of multiple sites: Application of the ligase chain reaction using PCR products instead of oligonucleotides", Biotechniques, 1993, 15(1), 69-71, 73-75, and 77.
PCT International Search Report dated Aug. 27, 1997, 1 page.
Hoshino et al., "Determination of the transcriptional initiation sites of the kanamycin resistance genes of pUB110 and pTHN1", J. Ferment. Bioeng. 79(1): 62-63, 1995.
McKenzie et al., "The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation", Plasmid 15 (2):93-103, Mar. 1986.
Holmes, D. et al., "Cloning of an Aminoglycoside-Resistance-Encoding Gene, KamC, from Saccharopolyspora hirsuta: Comparison with KamB from Streptomyces tenebrarius", Gene 1991, 102, 19-26.
Kues, U. and Stahl, "Replication of Plasmids in Gram-Negative Bacteria", Microbiological Reviews 1989, 53(4), 491-516.
Matsumura, M. et al., "Enzymatic and Nucleotide Sequence Studies of a Kanamycin-Inactivating Enzyme Encoded by a Plasmid from Thermophilic Bacilli in Comparison with That Encoded by Plasmid pUB110", J. of Bacteriol. 1984, 160(1), 413-420.
Oka, A. et al., "Nucleotide Sequence of the Kanamycin Resistance Transposon Tn903", J. Mol. Biol. 1981, 147, 217-226.
Sadaie, Y. et al., "Purification and Characterization of a Kanamycin Nucleotidyltransferase from Plasmid pUB110-Carrying Cells of Bacillus subtilis", J. of Bacteriol. 1980, 141(3), 1178-1182.
Satishchandran, C. et al., "Novel Escherichia coli K-12 Mutants Impaired in S-Adenosylmethionine Synthesis", J. of Bacteriol. 1990, 172(8), 4489-4496.
Schwotzer, U. et al., "R-Plasmid Mediated Aminoglycoside Resistance in Staphylococcus epidermidis: Structure Determination of the Products of an Enzyme Nucleotidylating the 4'- and 4"-Hydroxyl Group of Aminoglycoside Antibiotics", FEMS Microbiology Letters 1978, 3, 29-33.
Shaw, K.J. et al., "Correlation between Aminoglycoside Resistance Profiles and DNA Hybridization of Clinical Isolates", Antimicrobial Agents and Chemotherapy 1991, 35(11), 2253-2261.
Shaw, K.J. et al., "Molecular Genetics of Aminoglycoside Resistance Genes and Familial Relationships of the Aminoglycoside-Modifying Enzymes", Microbiol. Reviews 1993, 57(1), 138-163.
Siregar, J. et al., "Purification, Characterization, and Investigation of the Mechanism of Aminoglycoside 3'- Phosphotransferase Type Ia", Biochemistry 1995, 34, 12681-12688.
Wingard, Jr. L. et al., "Inhibitors of Bacterial Ribosomal Actions", Chap. 47 in Human Pharmacology Molecular-to-Clinical, Mosby Year Book, St. Louis, 1991, pp. 659-676.

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