Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1998-04-01
2000-09-19
Smith, Lynette R. F.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
4352523, 4353201, 435419, 536 2371, 800302, A01H 500, C12N 121, C12N 514, C12N 1532, C12N 1582
Patent
active
06121521&
ABSTRACT:
The present invention describes the design and construction of a chimeric insecticidal protein by joining the 5' portion of a synthetic maize optimized cry1B gene (SFLIB) to the 3' end of a full-length synthetic maize optimized cry1A(b) gene to generate a full-length hybrid cry1B gene (hyFLIB). When the chimeric insecticidal protein gene is expressed in transgenic maize from both PEPC and pith promoters, insecticidal activity is observed in transgenic maize tissue against European corn borer (Ostrinia nubilalis). An additional aspect of the invention is recombinant, biologically pure microbial strains transformed with the hyFLIB gene which can be used in entomocidal formulations for the control of Lepidopteran insects. Yet another aspect of the invention is plants transformed with the toxin gene or active fragments thereof, particularly where the transforming sequences have been optimized for expression in maize.
REFERENCES:
patent: 5380831 (1995-01-01), Adang et al.
patent: 5436391 (1995-07-01), Fujimoto et al.
patent: 5593881 (1997-01-01), Thompson et al.
patent: 5625136 (1997-04-01), Koziel et al.
patent: 5628995 (1997-05-01), Peferoen et al.
patent: 5736131 (1998-04-01), Bosch et al.
Fischhoff, et al., Bio/Technology 5: 807-813 (1987).
Murray, et al., Nucleic Acids Research 17(2): 477-498 (1989).
Vaeck, et al., Nature 328: 33-37 (1987).
Barton et al., Plant Physiol. (1987) 85, 1103-1109.
Brizzard and Whitely, Nucleic Acids Research, vol. 16, No. 6, 1988, 2723-2724.
Geiser et al., Gene, 48 (1986) 109-118.
Hofte and Whitely, MicroBiological Reviews, Jun. 1989, 242-255.
Koziel et al, Bio/Technology, vol. 11, Feb. 1993, 194-200.
Ohta et al., Mol Gen Genet, (1991) 225:369-378.
Perlak et al., Proc. Natl. Acad. Sci., USA, vol. 88, 3324-3328, Apr. 1991.
Meigs J. Timothy
Nelson Amy J.
Novartis AG
Pace Gary M.
Smith Lynette R. F.
LandOfFree
Chimeric insecticidal protein and DNA coding therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chimeric insecticidal protein and DNA coding therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chimeric insecticidal protein and DNA coding therefor will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1075102