Chimeric GFP-aequorin as bioluminescent Ca++reporters at the...

Chemistry: analytical and immunological testing – Element or inorganic compound

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S968000, C436S172000, C530S300000, C530S324000, C530S326000, C930S310000

Reexamination Certificate

active

06936475

ABSTRACT:
A modified bioluminescent system comprising a fluorescent molecule covalently linked with a photoprotein, wherein said link between the two proteins has the function to stabilize the modified bioluminescent system and allowing the transfer of the energy by Chemiluminescence Resonance Energy Transfer (CRET).

REFERENCES:
patent: 5614184 (1997-03-01), Sytkowski et al.
patent: 5714666 (1998-02-01), Pritchett et al.
patent: 2003/0066095 (2003-04-01), Baubet et al.
Baubet et al., Chimeric green flourescent protein-aequorin as bioluminescent Ca2+reporters at the single-cell level. Proc. Nat'l Acad. Sci. , vol. 97, pp. 7260-7265 (2000).
Chiesa, et al. Recombinant aequorin and green flourescent protein as valuable tools in the study of cell signalling. Biochem. J., vol. 355, pp. 1-12 (2001).
Miyawaki, et al. Dynamic and quantitative Ca2+measurements using improved cameleons, Proc. Nat'l Acad. Sci., vol. 96, pp. 2135-2140 (1999).
Miyawaki et al.., Flourescent indicators for Ca2+based on green flourescent proteins and calmodulin. Nature, vol. 388, pp. 882-887 (1997).
Pinton et al., New light on mitochondrial calcium, BioFactors, vol. 8, pp. 243-253 (1998).
Rutter et al. Subcellular imaging of intramitochondrial Ca2+with recombinant targeted aequorin: Significance for the regulation of pyruvate dehydrogenase activity. Proc. Nat'l Acad. Sci., vol. 93, pp. 5489-5494 (1996).
Campbell, et al., “Chemiluminescence Energy Transfer,”Principles and Application in Biology and Medicine, eds.474-534 (1988).
Campbell, et al., “Luminescence in Cells and Vesicles Isolated from the Hydroid Obelia Geniculata,”.Proc. Physiol. Soc., 287:4-5 (1978).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Chimeric GFP-aequorin as bioluminescent Ca++reporters at the... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chimeric GFP-aequorin as bioluminescent Ca++reporters at the..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chimeric GFP-aequorin as bioluminescent Ca++reporters at the... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3448883

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.