Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1995-06-07
1998-06-30
McElwain, Elizabeth F.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800250, 536 231, 536 236, 536 237, 536 241, 435 691, 4351723, 4353201, 435119, A01H 500, A01H 510, C12N 1582
Patent
active
057736910
ABSTRACT:
This invention relates to four chimeric genes, a first encoding lysine-insensitive aspartokinase (AK), which is operably linked to a plant chloroplast transit sequence, a second encoding lysine-insensitive dihydrodipicolinic acid synthase (DHDPS), which is operably linked to a plant chloroplast transit sequence, a third encoding a lysine-rich protein, and a fourth encoding a plant lysine ketoglutarate reductase, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine or threornine in the seeds of transformed plants are provided. Also provided are transformed plants wherein the seeds accumulate lysine or threonine to higher levels than untransformed plants.
REFERENCES:
patent: 5367110 (1994-11-01), Galili
Mertz, E., Science, 145, 279-280, 1964.
Nelson, Science, 150, 1469-1470, 1965.
Deutscher, D. Adv. Exp. Medicine and Biology, 105, 281-300, 1978.
Vasal, S.K., et al., Proceedings of the 3rd Seed Protecin Symposium, Gatersleben, Aug. 31-Sep. 2, 1983.
Giovanelli, J. et al, Plant Physiol, 90, 1584-1599, 1989.
Shaul, O. et al, Plant Journal, 2, 203-209, 1992.
Perl et al, Plant Mol. Biol., 19, 815-823, 1992.
Shaul, O., Plant Physiol., 100, 1157-1163, 1992.
Brochetto-Braga, M.R. et al, Plant Physiol., 98, 1139-1147, 1992.
Cassan, M. et al, Journal of Biological Chemistry, 26(3), Jan. 25, 1986.
Doyle, JJ. et al, J. Biol. Chem., 261, 9228-9236, 1986.
Altenbach, et al, Plant Mol. Biol., 8, 239-250, 1987.
Altenbach, et al, Plant Mol. Biol., 13, 513-522, 1989.
Glassman, et al, PCT Patent Application PCT/US89/01309, 1989.
Galili, et al, Abstr. 422 from Third Int'l Cong. of Int. Soc. Plant Mol. Biol., 1991.
Richard, et al, J. Bacteriology, 166, 297-300, 1986.
Bonnassie, et al, Nucleic Acids Research, 18, 6421, 1990.
Yeh, et al, Mol. Gen. Genet., 212, 105-111, 1988.
Katinka et al, Proc. Nat'l Acad. Sci. USA, 77, 5730-5733, 1980.
Zakin et al, J. Biol. Chem., 258, 3028-3031, 1983.
Rafalski et al, J. Biol. Chem., 263, 2146-2151, 1988.
Bright et al., Nature, 299, 278-279, 1982.
Rognes, et al, Planta, 157, 32-38, 1983.
Arruda, et al, Plant Physiol., 76, 442-446, 1984.
Hibbard, et al, Planta, 148, 183-187, 1980.
Diedrich, et al, Theor. Appl. Genet., 79, 209-215, 1990.
Dotson, et al, Planta, 182, 546-552, 1990.
Frankard, et al, Theor. Appl. Genet., 82, 273-282, 1991.
Wilson et al. Plant Physiol., 97, 1323-1328, 1991.
Kaneko, et al, J. Biol. Chem., 265, 17451-17455, 1990.
Frisch, et al, Mol. Gen. Genet., 228, 287-293, 1991.
Cassan, et al., J. Biol. Chem., 261, 1052-1057, 1986.
Negrutui et al, Theor. Appl. Genet., 68, 11-20, 1984.
Theze, et al, J. Bacteriol., 117, 133-143, 1974.
Giovanelli, et al, Plant Physiol., 90, 1577-1583, 1989.
Karchi, H. et al., Lysine synthesis and catabolism are coordinately regulated during tobacco seed development, Proc. Natl. Acad. Sci. USA, Plant Biology, 91, 2577-2581, Mar. 1994.
Pisabarro, A. et al., A Cluster of Three Genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum Encodes Dihydrodipicolinate Synthase, Dihydrodipicolinate Reductase, and a Third Polypeptide of Unknown Function, Journal of Bacteriology, 175, No. 9, 2743-2749, May 1993.
Ghislain, M. et al., A dinucleotide mutation in dihydrodipicolinate synthase of Nicotiana sylvestris leads to lysine overproduction, The Plant Journal, 8(5), 733-743, 1995.
Silk, Gregg W. et al., Cloning and expression of the soybean DapA gene encoding dihydrodipicolinate synthase, Plant Molecular Biology, 26, 289-993, 1994.
Brinch-Pedersen, Henrick et al., Engineering of the aspartate family biosynthetic pathway in barley (Hordeum vulgare L.) by transformation with heterologous genes encoding feed-back-insensitive aspartate kinase and dihydrodipicolinate synthase, Plant Molecular Biology, 32, 611-620, 1996
Bonnassie et al (1990) Nuclear Acids Res. 18(21):6421.
Falco Saverio Carl
Keeler Sharon Jo
Rice Janet Ann
E. I. Du Pont de Nemours and Company
McElwain Elizabeth F.
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