Chimeric genes and methods for increasing the lysine and...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S278000, C800S287000

Reexamination Certificate

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07071383

ABSTRACT:
This invention relates to four chimeric genes, a first encoding lysine-insensitive aspartokinase (AK), which is operably linked to a plant chloroplast transit sequence, a second encoding lysine-insensitive dihydrodipicolinic acid synthase (DHDPS), which is operably linked to a plant chloroplast transit sequence, a third encoding a lysine-rich protein, and a fourth encoding a plant lysine ketoglutarate reductase, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine or threonine in the seeds of transformed plants are provided. Also provided are transformed plants wherein the seeds accumulate lysine or threonine to higher levels than untransformed plants.

REFERENCES:
patent: 4886878 (1989-12-01), Larkins et al.
patent: 5024944 (1991-06-01), Collins et al.
patent: 5367110 (1994-11-01), Galili et al.
patent: 0 435 132 (1991-07-01), None
patent: 0 485 970 (1991-11-01), None
patent: WO 89/11789 (1989-12-01), None
patent: 8911789 (1989-12-01), None
patent: WO 93/1910 (1993-09-01), None
patent: WO 95/15392 (1995-06-01), None
patent: WO 95/34672 (1995-12-01), None
patent: WO 96/19588 (1996-06-01), None
Doyle, J.J. et al. (1986) J. Biol. Chem 261:9228-9236.
Casan et al (1986) J. Biol Chem 26(3):1052-1057.
Mertz (1964) Science 145:279-280.
Brochetto-Braga et al (1992) Plant Physiol 98:1139-1147.
Guerde et al (1990) Mole Gen Genet 221 306-314.
Jacobs et al (1985) Nature 313:806-810.
Pedersen et al (1986) J Biol Chem 261(14):6279-6284.
Leonard et al (1984) Nature 311:626-631.
Matsudaira (1987) J Biol Chem 262(21):10035-10038.
Matsudaira (1990) Methods in Enzymol. 182:602-613.
Lewin (1987) Science 237:1570.
Bonnassie et al (1990) Nucleic Acids Res. 18(21):6421.
Mertz, E.,Science, 145, 279-280, 1964.
Nelson,Science, 150, 1469-1470, 1965.
Deutscher, D.Adv. Exp. Medicine and Biology, 105, 281-300, 1978.
Vasal, S.K., et al., Proceedings of the 3rd Seed Protecin Symposium, Gatersleben, Aug. 31-Sep. 2, 1983.
Giovanelli, J. et al,Plant Physiol, 90, 1584-1599, 1989.
Shaul, O. et al,Plant Journal, 2, 203-209, 1992.
Perl et al,Plant Mol. Biol., 19, 815-823, 1992.
Shaul, O.,Plant Physiol., 100, 1157-1163, 1992
Brochetto-Braga, M.R. et al,Plant Physiol., 98, 1139-1147, 1992.
Doyle, JJ. et al,J. Biol. Chem., 261, 9228-9236, 1986.
Altenbach, et al,Plant Mol. Biol., 8, 239-250, 1987.
Altenbach, et al,Plant Mol. Biol., 13, 513-522, 1989.
Glassman, et al, PCT Patent Application PCT/US89/01309, 1989.
Galili, et al, Abstr. 422 fromThrid Int'l Cong. of Int. Soc. Plant Mol. Biol., 1991.
Richard, et al,J. Bacteriology, 166, 297-300, 1986.
Bonnassie, et al,Nucleic Acids Research, 18, 6421, 1990.
Yeh, et al,Mol. Gen. Genet., 212, 105-111, 1988.
Katinka et al,Proc. Nat'l. Acad. Sci. USA, 77, 5730-5733, 1980.
Zakin et al,J. Biol. Chem., 258, 3028-3031, 1983.
Rafalski et al,J. Biol. Chem., 263, 2146-2151, 1988.
Bright, et al,Nature, 299, 278-279, 1982.
Rognes, et al,Planta, 157, 32-38, 1983.
Arruda, et al,Plant Physiol., 76, 442-446, 1984.
Hibbard, et al,Planta, 148, 183-187, 1980.
Diedrich, et al,Theor. Appl. Genet., 79, 209-215, 1990.
Dotson, et al,Planta, 182, 546-552, 1990.
Frankard, et al,Theor. Appl. Genet., 82, 273-282, 1991.
Wilson, et al.Plant Physiol., 97, 1323-1328, 1991.
Kaneko, et al,J. Biol. Chem., 265, 17451-17455, 1990.
Frisch, et al,Mol. Gen. Genet., 228, 287-293, 1991.
Cassan, et al.J. Biol. Chem., 261, 1052-1057, 1986.
Negrutui et al,Theor. Appl. Genet., 68, 11-20, 1984.
Theze, et al,J. Bacteriol., 117, 133-143, 1974.
Giovanelli, et al,Plant Physiol., 90, 1577-1583, 1989.
Karchi, H. et al., Lysine synthesis and catabolism are coordinately regulated during tobacco seed development, Proc. Natl. Acad. Sci. USA, Plant Biology, 91, 2577-2581, Mar. 1994.
Ghislain, M. et al., A dinucleotide mutation in dihydrodipicolinate synthase of Nicotiana sylvestris leads to lysine overproduction, The Plant Journal, 8(5), 733-743, 1995.
Silk, Gregg W. et al., Cloning and expression of the soybean DapA gene encoding dihydrodipicolinate synthase, Plant Molecular Biology, 26, 289-993, 1994.
Brinch-Pedersen, Henrick et al., Engineering of the aspartate family biosynthetic pathway in barley (Hordeum vulgare L.) by transformation with heterologous genes encoding feed-back-insensitive aspartate kinase and dihydrodipicolinate synthase, Plant Molecular Biology, 32, 611-620, 1996.
Pisabarro, A. et al., A Cluster of Three Genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum Encodes Dihydrodipicolinates Synthase, Dihydrodipicolinate Reductase, and a Third Polypeptide of Unknown Function, Journal of Bacteriology, 175, No. 9, 2743-2749, May 1993.
Arruda, P. et al., Lysine-Ketoglutarate Reductase Activity in Developing Maize Endosperm, Plant Physiology, vol., 69, pp. 988-989, 1982.
National Center for Biotechnology Information General Identifier No. 2052507, Accession No. U95759, Jan. 16, 1998, S. Epelbaum et al.

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