Chimeric gene for the transformation of plants

Details Chimeric gene for the transformation of plants Chimeric gene for the transformation of plants

1994-05-31

1996-04-23

Fox, David T.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 236, 435 691, 4351723, 435 697, C07H 1700, C12N 1500, C12N 1529, C12N 1582

Patent

active

055104711

ABSTRACT:
Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyadenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localised enzyme, a partial sequence of the N-terminal mature part of a plant gene encoding a plastid-localised enzyme and then a second transit peptide of a plant gene encoding a plastid- localised enzyme. Production of glyphosate-tolerant plants is disclosed.

REFERENCES:
Smeekens et al. (1990) TIBS 15: 73-76.
LeBrun et al. (1987) Nucl. Acids Res. 15(10): 4360.
Waksman et al. (1987) Nucl. Acids Res. 15(17): 7181.
L. Comai, et al. (1988) The Journal of Biological Chemistry vol. 263, No. 29 Issue of Oct. 15, pp. 15104-15109, "Chloroplast Transport of a Ribulose Bisphosphate Carboxylase Small Subunit-5-Enolpyruvyl 3-Phosphoshikimate Synthase Chimeric Protein Requires Part of the Mature Small Subunit in Addition to the Transit Preptide".

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