Chimeric fused monooxygenase of cytochrome P-450 and NADPH-cytoc

Details Chimeric fused monooxygenase of cytochrome P-450 and NADPH-cytoc Chimeric fused monooxygenase of cytochrome P-450 and NADPH-cytoc

1990-03-27

1992-05-19

Patternson, Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

430401, 435 27, 435 47, C12N 902, C07K 1300

Patent

active

051148528

ABSTRACT:
The present invention provides a chimeric enzyme gene which codes for a monooxygenase having both monooxygenase activity derived from cytochrome P-450 and reducing power supplying ability derived from NADPH-cytochrome P-450 reductase.
The present invention further provides a yeast expression plasmid which contains said chimeric enzyme gene and expresses said monooxygenase gene; a transformed yeast strain which carries said yeast expression plasmid; a monooxygenase which has both the monooxygenase activity and the reducing power supplying ability as mentioned above; and a process for producing said monooxygenase.

REFERENCES:
patent: 4766068 (1988-08-01), Oeda et al.
patent: 4774180 (1988-09-01), Toth et al.
Genetically Engineered Modification of P450 Monooxygenases: . . . Enzyme, Yabushiki et al., DNA, vol. 7, No. 10, 1988, pp. 701-711.
Oeda, K., et al., (1985), DNA 4(3), 203-210.
Murakami, H., et al., (1986), DNA 5(1), 1-10.
Yabusaki, Y., et al., (1984), J. Biochem., 96, 793-804.
Yabusaki, Y., et al., (1984), Nucl. Acids Res., 12(6), 2929-2938.
Narhi, L. O., et al., (1986), J. Biol. Chem., 261(16), 7160-7169.

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