Chimeric fused monooxygenase of cytochrome P-450 and NADPH-cytoc

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

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4352542, 4353201, 435189, 435 697, 536 232, 530401, C12N 118, C12N 1581, C12N 1553

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054361591

ABSTRACT:
The present invention provides a chimeric enzyme gene which codes for a monooxygenase having both monooxygenase activity derived from cytochrome P-450 and reducing power supplying ability derived from NADPH-cytochrome P-450 reductase.
The present invention further provides a yeast expression plasmid which contains said chimeric enzyme gene and expresses said monooxygenase gene; a transformed yeast strain which carries said yeast expression plasmid; a monooxygenase which has both the monooxygenase activity and the reducing power supplying ability as mentioned above; and a process for producing said monooxygenase.

REFERENCES:
Yabusaki, Y., et al. (1988) DNA 7(10), 701-711.
Porter, T. D., et al. (1985) Proc. Natl. Acad. Sci., USA 82, 972-977.
Black, S. D., et al. (1982) J. Biol. Chem. 257(10), 5929-5938.

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