Chimeric filovirus glycoprotein

Details Chimeric filovirus glycoprotein Chimeric filovirus glycoprotein

2002-01-31

2010-06-08

Parkin, J. S. (Department: 1648)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Combination of antigens from multiple viral species

C424S204100, C435S320100

Reexamination Certificate

active

07731975

ABSTRACT:
Chimeric GP molecules were constructed which contain portions of both the EBOV and MBGV GP proteins by swapping the subunits between EBOV and MBGV. The chimeric molecules were cloned into an alphavirus replicon which offers the advantage of high protein expression levels in mammalian cells and is a proven vaccine vector. These chimeric molecules fully protected guinea pigs from MBGV challenge, and conversely protected the animals from EBOV challenge. These results indicate that a protective epitope resides within the GP2 subunit of the MBGV GP protein and at least partially within the GP2 subunit of the EBOV GP protein. Additionally these results show that a construction of a single-component bivalent vaccine protective in guinea pigs is achievable.

REFERENCES:
patent: WO0000616 (2000-01-01), None
patent: WO0000617 (2000-01-01), None
Partidos, C., et al., 1992(a), “The effect of orientation of epitopes on the immunogenicity of chimeric synthetic peptides representing measles virus protein sequences.”, Mol. Immunol. 29(5):651-8 (abstract provided).
Partidos, C., et al., 1992(b), “The effects of a flanking sequence on the immune response to a B and a T cell epitope from the fusion protein of measles virus.”, J. Gen. Virol. 73(8):1987-94 (abstract provided).
Janssen, R., et al., 1994, “Influence of amino acids of a carrier protein flanking an inserted T cell determinant on T cell stimulation.”, Internat. Immunol. 6(8):1187-1193 (abstract provided).
Eisenlohr, L. C., et al., 1992, “Flanking sequences influence the presentation of an endogenously synthesized peptide to cytotoxic T lymphocytes.”, J. Exp. Med. 175(2):481-7 (abstract provided).
Ayato, T., et al., 2003, “Identification of protective epitopes on Ebola virus glycoprotein at the single amino acid level by using recombinant vesicular stomatitis viruses.”, J. Virol. 77(2):1069-74 (abstract provided).
London, S. D. et al., 1992. Infectious enveloped RNA virus antigenic chimeras. PNAS, 89, 207-211.
Klenk Hans Dieter et al., 2001. Symposium on Marburg and Ebola viruses. Virus Research 80, 117-123.

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