Chimeric enzyme for promoting targeted integration of foreign DN

Details Chimeric enzyme for promoting targeted integration of foreign DN Chimeric enzyme for promoting targeted integration of foreign DN

1995-05-09

2000-11-21

Jones, W. Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 243, C07H 2102, C07H 2104

Patent

active

061505110

ABSTRACT:
The present invention provides novel chimeric and fusion proteins useful for facilitating site-specific integration of foreign DNA into a host genome. The chimeric enzymes of the invention comprise a DNA binding moiety fused to a retroviral integrase moiety, preferably at the precise N- or C-terminus. Nucleic acids encoding these fusion proteins can be incorporated into standard retroviral vectors, or can be provided as purified proteins. They are capable of exerting the activities of a wildtype retroviral integrase, including processing retroviral DNA termini, nicking double-stranded DNA and integrating a DNA molecule with processed retroviral termini into another DNA strand.

REFERENCES:
patent: 5532143 (1996-07-01), Grosveld et al.
patent: 5830707 (1998-11-01), Bushman
Devine et al. Nucleic Acids Research 22(18): 3765-3772 (1994).
Pahl et al. Journal of Biological Chemistry 270(7): 2957-2966 (1995).
Woerner et al. Nucleic Acids Research 21(15): 3507-3511.
Bushman, PNAS 91: 9233-9237 (Sep. 1994).
Boris-Lawrie and Temin, Curr. Op. Genet. Devel. 3:102-109 (1993).
Bouck et al., Mol. Cell. Biol. 15:2663-2671 (1995).
Brent and Ptashne, Cell 43:729-736 (1985).
Burley, Curr. Op. Struct. Biol. 4:3-11 (1994).
Chow et al., Science 255:723-726 (1992).
Doolittle et al., Quart. Rev. Biol. 64:1-13 (1989).
Garfinkle, The Retroviridae, vol. 1, Ch. 4, pp. 107-158. J.A. Levy ed., Plenum Press, NY (1992).
Horii et al., Cell 23:689-697 (1981).
Katz and Skalka, Ann. Rev. Biochem. 63:133-173 (1994).
Katz and Skalka, Mol. Cell. Biol. 10:696-704 (1990).
Katzman et al., J. Virol. 63:5319-5327 (1989).
Kulkosky et al. Virology 206:448-456 (1995).
Latchman, Eucaryotic Transcription Factors, Academic Press, Inc., San Diego, CA (1991).
McNeil et al., J. Cell Biol. 98:1556-1564 (1984).
Pryciak et al., EMBO J. 11:291-303 (1992).
Terry et al., J. Virology 62:2358-2365 (1988).
Bushman and Wang, J. Virol. 68:2215-2223 (1994).
Stewart and Vogt, J. Virol. 65:6281-6231 (1991).

Affiliated with

Also associated with

Rating

Chimeric enzyme for promoting targeted integration of foreign DN does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chimeric enzyme for promoting targeted integration of foreign DN, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chimeric enzyme for promoting targeted integration of foreign DN will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1258615