Chimeric envelope proteins for viral targeting

Details Chimeric envelope proteins for viral targeting Chimeric envelope proteins for viral targeting

1993-11-04

1998-02-03

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 4353201, 4352401, 4352402, 435 691, 435 697, 536 231, 536 234, C12Q 168, C12Q 170, C07H 2102, C12N 1500

Patent

active

057143161

ABSTRACT:
The present invention provides compositions and methods for targeting recombinant retroviral particles specifically to cells of interest for delivery of desired therapeutic or toxic agents. The invention provides chimeric nucleotide constructs, chimeric proteins formed of a selected viral envelope gene from which a selected sequence has been deleted and into which has been inserted all or an effective portion of a heterologous ligand, said ligand or portion thereof capable of binding to a selected receptor, recombinant viral particles formed of the chimeric proteins, a biological mediator for delivery to the target cell; and retroviral gag and pol proteins. The lack of retroviral nucleic acid renders the viral particle replication defective and non-pathogenic.

REFERENCES:
patent: 4593002 (1986-06-01), Dulbecco
R. Tellier et al. (1985) Nature 318:414.
L. A. Lasky et al. (1987) Cell 50:975-985.
A. W. Nienhuis et al. (1991) Cancer 67:2700-2704.
E. O. Freed et al (1990) Proc. Natl. Acad. Sci. USA 87:4650-4654.
S. A. Cannistra et al (1990) Proc. Natl. Acad. Sci. USA 87:93-97.
Cleland, In Protein Folding:In Vivo and In Vitro, ACS, Washington, D.C., CH 1 and CH 19, 1993.
Tellier et al. Nature 318: 414, 1985.
Laskey et al. Cell 50: 975-985, 1987.
Freed et al. PNAS 87: 4650-4654, 1990.
Guitierrez et al. The Lancet 339: 715-721, 1992.
Orkin et al. Report and Recommendations of the PAnel to Assess the NIH Investmetn in Research on Gene Therapy (volume not relevant), 1995.
N. Kasahara et al, "Tissue-Specific Targeting of Retroviral Vectors Through Ligand-Receptor Interactions", Science, 266:1373-1376 (Nov. 25, 1994).
T-H. Chu et al, "Retroviral Vector Particles Displaying the Antigen-Binding Site of an Antibody Enable Cell-Type-Specific Gene Transfer", J. Virol., 69(4):2659-2663 (Apr., 1995).
P. Correll et al, "Expression of Human Glucocerebrosidase in Long-Term Reconstituted Mice Following Retroviral-Mediated Gene Transfer into Hematopoietic Stem Cells", Human Gene Therapy, 1:277-287 (1990).
J. Wilson et al, "Expression of Human Adenosine Deaminase in Mice Reconstituted with Retrovirus-Transduced Hematopoietic Stem Cells", Proc. Natl. Acad. Sci. USA, 87:439-443 (Jan., 1990).
O. Danos et al, "Safe and Efficient Generation of Recombinant Retroviruses with Amphotropic and Ecotropic Host Ranges", Proc. Natl. Acad. Sci. USA, 85:6460-6464 (Sep., 1988).
J. Young et al, "Efficient Incorporation of Human CD4 Protein in Avian Leukosis Virus Particles", Science, 250:1421-1423 (Dec. 7, 1990).

Affiliated with

Also associated with

Rating

Chimeric envelope proteins for viral targeting does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chimeric envelope proteins for viral targeting, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chimeric envelope proteins for viral targeting will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-661184