Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2003-05-19
2008-08-05
Bragdon, Kathleen Kerr (Department: 1656)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C424S185100, C435S320100, C435S235100
Reexamination Certificate
active
07407807
ABSTRACT:
The invention describes a method for producing a chimaeric human papillomavirus (HPV) L1 polypeptide containing a heterologous peptide, and in particular, a HPV L2 peptide. The method comprises the steps of introducing a DNA sequence coding for the heterologous peptide into a DNA sequence coding for the L1 polypeptide; introducing the DNA sequence including the sequences for the L1 polypeptide and heterologous peptide into a host cell in which the DNA sequence can be expressed; causing expression of the DNA sequence; and recovering the resulting chimaeric L1 polypeptide which includes the heterologous peptide. Typically, the nucleotides encoding the heterologous peptide replace the nucleotides of the L1 polypeptide at the point of insertion. The invention also describes a vector for use in the method, a host cell containing the vector, and a vaccine including the chimaeric HPV L1 polypeptide produced according to the method.
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Kimbauer et al. (2001) Papillomavirus L1 major capsid protein self-assembles into virus-like particles that are highly immunogenic, Proc. Natl. Acad. Sci. U S A., vol. 89, No. 24, pp. 12180-12184.
Mueller, et al., “Chimeric Papillomavirus-like Particles”, Virology, United States, vol. 234, No. 1, pp. 93-111, Jul. 21, 1997.
International Search Report (Form PCT/ISA210), 2 pgs., International Application No. PCT/IB03/01912; dated Jan. 5, 2004.
Rybicki Edward Peter
Varsani Arvind Devshi
Bragdon Kathleen Kerr
Liu Samuel Wei
Norris McLaughlin & Marcus PA
University of Cape Town
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