Chemotactic test apparatus and method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435285, 435300, 435301, 422101, C12Q 102, C12M 304, C12M 118, C12M 120, B01L 1100

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active

053025156

ABSTRACT:
The present invention is a single site or multiple site chemotaxis test apparatus or chemotaxis chamber for measuring the effect of concentration gradients of diffusible chemicals upon the directional response of biological cells. The chemotactic test apparatus of the present invention comprise two compartments separated by a thin passage. The dimensions of the thin passage are selected such that cells cannot flow through the thin passage, but must deform themselves by flattening out and then "crawl" or migrate through the passage. These chambers can be used to study the migration or orientation of mobile adherent or non-adherent cells.

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B. Gatewood, J. Joe and S. H. Zigmond, "CD45 is not Involved in the Processing of Spatial Information Required for Chemotaxis," Journal of Immunology, vol. 147, pp. 243-246.
Falk, W., Goodwin, Jr., R. H. and Leonard, E. J., "A 48-Well Micro Chemotaxis Assembly for Rapid and Accurate Measurement of Leukocyte Migration," Journal of Immunological Methods, 33, 1980, pp. 239-247.
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