Chemotactic selection of pollutant degrading soil bacteria

Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Destruction of hazardous or toxic waste

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435 34, 435 39, 4352625, 435264, 435281, 435821, C12Q 104, C12Q 100, C12R 101

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active

053246616

ABSTRACT:
A method for identifying soil microbial strains which may be bacterial degraders of pollutants comprising the steps of placing a concentration of a pollutant in a substantially closed container, placing the container in a sample of soil for a period of time ranging from one minute to several hours, retrieving the container, collecting the contents of the container, and microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to inoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

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McCarty J of Hazardous Materials 28 (1991) pp. 1-11.
Fard et al Biotechnology & Bioengineering vol 37 pp. 661-672 Mar. 1991.

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