Chemoselective ligation

Details Chemoselective ligation Chemoselective ligation

2011-03-08

2011-12-13

Witherspoon, Sikarl (Department: 1621)

Organic compounds -- part of the class 532-570 series

Organic compounds

Azides

C552S011000, C552S012000, C564S123000, C435S007200

Reexamination Certificate

active

08076496

ABSTRACT:
The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, the invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).

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