Chemometric analysis for extraction of individual fluorescence s

Details Chemometric analysis for extraction of individual fluorescence s Chemometric analysis for extraction of individual fluorescence s

1997-10-27

1999-09-21

Hannaher, Constantine

Radiant energy

Luminophor irradiation

2504591, G01N 2164

Patent

active

059557370

ABSTRACT:
The present invention is a system for chemometric analysis for the extraction of the individual component fluorescence spectra and fluorescence lifetimes from a target mixture. The present invention combines a processor with an apparatus for generating an excitation signal to transmit at a target mixture and an apparatus for detecting the emitted signal from the target mixture. The present invention extracts the individual fluorescence spectrum and fluorescence lifetime measurements from the frequency and wavelength data acquired from the emitted signal. The present invention uses an iterative solution that first requires the initialization of several decision variables and the initial approximation determinations of intermediate matrices. The iterative solution compares the decision variables for convergence to see if further approximation determinations are necessary. If the solution converges, the present invention then determines the reduced best fit error for the analysis of the individual fluorescence lifetime and the fluorescence spectrum before extracting the individual fluorescence lifetime and fluorescence spectrum from the emitted signal of the target mixture.

REFERENCES:
patent: 5196709 (1993-03-01), Berndt et al.
patent: 5528368 (1996-06-01), Lewis et al.
patent: 5548124 (1996-08-01), Takeshima et al.
Weber, G., Resolution of the Fluorescence Lifetimes in a Heterogeneous System by Phase and Modulation Measurements, J. Phys. Chem., 85, (1981) pp. 949-953.
Lackowicz, J.R., Analysis of Fluorescence Decay Kinetics from Variable-Frequency Phase Shift and Modulation Data, Biophys. J., 46 (1984) pp. 463-477.
Burdick, D.S. et al., Resolution of Multicomponent Fluorescent Mixtures by Analysis of the Excitation-Emission-Frequency Array, J. Chemometrics, 4 (1990) pp. 15-28.

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