Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 6 to 7 amino acid residues in defined sequence
Reexamination Certificate
2005-11-08
2005-11-08
Kemmerer, Elizabeth (Department: 1647)
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
6 to 7 amino acid residues in defined sequence
C530S327000, C514S002600, C514S015800, C514S017400
Reexamination Certificate
active
06962971
ABSTRACT:
This invention is based on the discovery that chemokines induce fibroblasts to differentiate to myofibroblasts, which play a critical role in wound healing and are implicated in a number of fibrotic diseases. This activity has been localized to a peptide in the N-terminus of several chemokines. Accordingly, the invention provides polypeptides that induce the differentiation of fibroblasts to myofibroblasts in vitro and in vivo, nucleic acids encoding such polypeptides and related vectors, host cells, and composition containing these components. The invention also encompasses methods for inducing or inhibiting differentiation of fibroblasts to myofibroblasts, in vivo as well as in vitro, and screening methods for identifying other agents that modulate myofibroblast differentiation.
REFERENCES:
patent: 5194596 (1993-03-01), Tischer et al.
patent: 5350836 (1994-09-01), Kopchick et al.
patent: 5401651 (1995-03-01), Walz
patent: 5641867 (1997-06-01), Stern et al.
patent: 5900235 (1999-05-01), Gosselin et al.
patent: 6103234 (2000-08-01), Wolpe et al.
patent: 11-21296 (1999-01-01), None
patent: 11-43445 (1999-02-01), None
Ngo et al., The Protein Folding Problem and Tertiary Structure Prediction, pp. 433-440 and 492-495 (1994).
Wells, Additivity of Mutational Effects in Proteins. Biochemistry 29:8509-8517 (1990).
Herbert et al. Scanning Mutagenesis of Interleukin-8 Identifies a Cluster of Residues Required for Receptor Binding. The Journal of Biological Chemistry, vol. 266/28 pp. 18989-18994 (1991).
Oppenheim, J Overview of Chemokines. Adv. Exp. Med. Biol. vol. 351 pp. 183-186 (1993).
Baggiolini, M Chemotactic and Inflammatory Cytokines-CXC and CC Proteins Adv. Exp. Med. Biol. vol. 351 pp. 1-11 (1993).
Murdoch et al. Chemokine receptors and their role in inflammation and infectious disease Blood vol. 95/10 May 15, 2000.
Well, JA Additivity of Mutational Effects in Proteins. Biochemistry 29:8509-8517 (1990).
Yan et al. Two-Amino Acid Molecular Switch in an Epithelial Morphogen That Regulates Binding to Two Distinct Receptors. Science 90:523-527 (2000).
Ngo et al. Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox. The Protein Folding Problem and Tertiary Structure Prediction. Merz and Le Grand, Editors, Birkhauser, Boston, 1994.
Baggiolini, M., Dewald, B., and B. Moser. 1997. Human chemokines: an update.Annu. Rev. Immunol. 15:675-705.
Bazan, J.F., Bacon, K.B., Hardiman, G., Wang, W., Soo, K., Rossi, D., Greaves, D.R., Zlotnik, A., and T.J. Schall. 1997. A new class of membrane-bound chemokine with a CX3C motif.Nature(Lond.) 385: 640-644.
Belperio, J.A., Keane, M., Arenberg, D., Addison, C., Ehlert, J., Burdick, M.D., and R. Strieter. 2000. CXC chemokines in angiogenesis.J. Leukoc. Biol. 68-:1-8.
Brown, L., Dubin, D., Lavigne, L., Logan, B., Dvorak, H., and L. Van de Water.1993. Macrophages and fibroblasts express embryonic fibronectins during cutaneous wound healing.Am. J. Pathol. 142:793-801.
Carmeliet, P. 2000. Mechanisms of angiogenesis and arteriogenesis.Nature Med. 6:389-95.
Clark, R. 1993. Basics of cutaneous wound repair. J. Dermatol. Surg. Oncol. 19:693-706.
Clark-Lewis, I., Kim K., Rajarathnam, K., Gong, J., Dewald, B., Moser, B., et al. 1995. Structure-activity relationships of chemokines. J Leukoc. Biol. 57:703-711.
Coffin, C., Dehner, L., and J. Meis-Kindblom. 1998 Inflammatory myofibroblastic tumor, inflammatory fibrosarcoma, and related lesions: an historical review with differential diagnostic considerations. Seminars in Diagnostic Pathology 15:102-110.
Desmouliere, A., Geinoz, A., Gabbiani, F., and G. Gabbiani. 1993. Transforming growth factor-beta 1 induces alpha-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J. Cell Biol. 122:103-111.
Devalaraja, R., Nanney, L., Qian, Q., Du, J., Yu, Y., Devalaraja, M.N., and A. Richmond. 2000. Delayed wound healing in CXCR2 knockout mice. J. Investig. Dermatol. 115:234-44.
Dimitrijevic-Bussod, M., Balzaretti-Maggi, V., and D. Gadbois. 1999. Extracellular matrix and radiation G1 cell cycle arrest in human fibroblasts. Cancer Res. 59:4843-4847.
Doucet, J., and J. Trifaro. 1988. A discontinous and highly porous sodium dodecyl sulfate-polyacrylamide slab gel system of high resolution. Anal. Biochem. 168:265-271.
Dunleavy, J., and J. Couchman. 1995. Interleukin-8 induces motile behavior and loss of focal adhesions in primary fibroblasts. J. Cell Sci. 108:311-321.
Engelhardt, E., Toksoy, A., Goebeler, M., Debus, S., Bröcker, E., and R. Gillitzer. 1998. Chemokines IL-8, GROalpha, MCP-1, IP-10, and Mig are sequentially and differentially expressed during phase-specific infiltration of leukocyte subsets in human wound healing. Amer. J. Pathol. 153:1849-1860.
Feugate, J, Li, Q., and Martins-Green, M. 2002. The cxc chemokine cCAF stimulates differentiation of fibroblasts into myofibroblasts and accelerates wound closure. J. of Cell Biology. 156:161-172.
Gabbiani, G. 1996. The cellular derivation and the life span of the myofibroblast. Pathol. Res. Pract. 192:708-711.
Germain, L., Jean, A., Auger, F., and D. Garrel. 1994. Human wound healing fibroblasts have greater contractile properties than dermal fibroblasts. J. Surg. Res. 57:268-273.
Gharaee-Kermani, M., Denholm, E., and S. Phan. 1996. Costimulation of fibroblast collagen and transforming growth factor b1 gene expression by monocyte chemoattractant protein-1 via specific receptors. J. Biol. Chem. 271:17779-17784.
Gupta, S. and J. Singh. 1994. Inhibition of endothelial cell proliferation by platelet factor-4 involves a unique action on S phase progression. J. Cell Biol. 127:1121-1127.
Hasegawa, M., Sato, S., and K. Takehara. 1999. Augmented production of chemokines (monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1 alpha (MIP-1alpha) and MIP-1beta) in patients with systemic sclerosis: MCP-1 and MIP-1alpha may be involved in the development of pulmonary fibrosis. Clin. Exp. Immunol. 117:159-165.
Jester, J., Huang, J., Barry-Lane, P., Kao, W., Petroll, W., and H. Cavanagh. 1999. Transforming growth factor(beta)-mediated corneal myofibroblast differentiation requires actin and fibronectin assembly. Invest. Ophthalmol. Vis. Sci. 40:1959-1967.
Kadono, T., Kikuchi, K., Ihn, H., Takehara, K., and K. Tamaki. 1998. Increased production of interleukin 6 and interleukin 8 in scleroderma fibroblasts. J. Rheumatol. 25:296-301.
Keane, M., Arenberg, D., Lynch, J., Whyte, R., Iannettoni,-M., Burdick, M., Wilke, C., Morris, S., Glass, M., DiGiovine, B., Kunkel, S., and R. Strieter. 1997. The CXC chemokines, IL-8 and IP-10, regulate angiogenic activity in idiopathic pulmonary fibrosis. J. Immunol. 159:1437-1443.
Khouw, I., van Wachem, P., Plantinga, J., Vujaskovic, Z., Wissink, M., de Leij, L., and M. van Luyn. 1999. TGF-beta and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro. Biomaterials 20:1815-1822.
Lanning, D., Diegelmann, R., Yager, D., Wallace, M., Bagwell, C., and J. Haynes. 2000. Myofibroblast induction with transforming growth factor-beta1 and -beta3 in cutaneous fetal excisional wounds. J. Pediatr. Surg. 35:183-187.
Luo, Y., D,Amore, P., and M. Dorf. 1996. b-chemokine TCA3 binds to and activates rat vascular smooth muscle cells. J. Immunol. 157:2143-2148.
Luster, A., Cardiff, R., MacLean, J., Crowe, K., and R. Granstein. 1998. Delayed wound healing and disorganized neovascularization in transgenic mice expressing the IP-10 chemokine. Proceedings of the Association of American Physicians 110:183-196.
Mackie, E., Halfter, W., and D. Liverani. 1988. Induction of tenascin in healing wounds. J. Cell Biol. 107:2757-2767.
Martins-Green, M., and M. Bissell. 1990. Localization of 9E3/CEF-4 in avian tissues: expression is absent in Rous sarcoma virus-induced tumors but is stimulated by injury. J. Cell. Biol. 110:581-595.
Martins-Green, M., Tilley, C., Schwarz, R., Hatier, C., and M. Bissell. 1991. Wound-factor-induced and cell cycle phase-dependent expression of 9E3/CEF4, the avian gro gene. Cell Regul. 2:739-52.
Martins-Green
Feugate Jo Ellen
Li QiJing
Martins-Green Manuela
DeBerry Regina M.
Haliday Emily M.
Kemmerer Elizabeth
Quine Intellectual Property Law Group P.C.
The Regents of the University of California
LandOfFree
Chemokines and methods for inducing the differentiation of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chemokines and methods for inducing the differentiation of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chemokines and methods for inducing the differentiation of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3471959