Chemokine-like proteins and methods of use

Details Chemokine-like proteins and methods of use Chemokine-like proteins and methods of use

1995-06-07

1998-08-04

Ulm, John

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

25 or more amino acid residues in defined sequence

424 852, 435 6952, 435 701, 435 711, 4351723, 514 2, 514 12, 514885, C07K 1454, A61K 3820, C12N 1524

Patent

active

057895392

ABSTRACT:
The invention relates to novel chemokine-like proteins that include two or more newly discovered active domains from different chemokines. Active domains are regions of several contiguous amino acids that are necessary for chemokines' ability to suppress the proliferation of actively dividing myeloid cells, e.g., myeloid progenitor cells, myeloid stem cells, and leukemic cells. The new chemokine-like proteins provide higher myelosuppressive activity than naturally occurring, wild-type chemokines.

REFERENCES:
patent: 5149544 (1992-09-01), Gentile et al.
patent: 5185323 (1993-02-01), Gewirtz
patent: 5294544 (1994-03-01), Gentile et al.
Beall et al., "Conversion of Monocyte Chemoattractant Protein-1 into a Neutrophil Attractant by Substitution of Two Amino Acids", J. Biol. Chem., 267 :3455-3459 (1992).
Broxmeyer et al., "Enhancing and Suppressing Effects of Recombinant Murine Macrophage Inflammatory Proteins on Colony Formation In Vitro by Bone Marrow Myeloid Progenitor Cells", Blood, 76 :1110-1116 (1990).
Broxmeyer et al., Macrophage Inflammatory Protein (MIP)-1.beta. Abrogates the Capacity of 1.alpha. To Suppress Myeloid Progenitor Cell Growth:, J. Immunol., 147 :2586-2594 (1991).
Broxmeyer et al., "Comparative Analysis of the Human Macrophage Inflammatory Protein Family of Cytokines (Chemokines) on Proliferation of Hyman Myeloid Progenitor Cells", J. Immunol., 150 :3448-3458 (1993).
Clark-Lewis et al., "Platelet factor 4 binds to interlukin 8 receptors and activates neutrophils when its N terminus is modified with Glu-Leu-Arg", Proc. Natl. Acad. Sci. USA, 90 :3574-3577 (1993).
Cooper et al., "Myelosuppressive effects in vivo with very low dosages of monomeric recombinant murine macrophage inflammatory protein-1.alpha.", Experimental Hematology, 22 :186-193 (1994).
Dunlop et al., "Demonstration of Stem Cell Inhibition and Myeloprotective Effects of SCI/rhMIP1.alpha. In Vivo", Blood, 79 :2221-2225 (1992).
Graham et al., "Identification and Characterization of an Inhibitor of Haemopoietic Stem Cell Proliferation" Nature, 344:442-444 (1990).
Gewirtz et al., "Inhibition of Human Megakaryocytopoiesis In Vitro by Platelet Factor 4 (PF4) and a Synthetic COOH-Terminal PF4 Peptide", J. Clin. Invest. 83 :1477-1486 (1989).
Han et al., "Platelet Factor 4 Inhibits Human Megakaryocytopoiesis In Vitro", Blood, 75 :1234-1239 (1990).
Hebert et al., "Scanning Mutagenesis of Interleukin-8 Identifies a Cluster of Residues Required for Receptor Binding", J. Biol. Chem., 266 :18989-18994 (1991).
Lord et al., "Macrophage-Inflammatory Protein Protects Multipotent Hematopoietic Cells from the Cytotoxic Effects of Hydroxyurea In Vivo", Blood, 79 :2605-2609 (1992).
Mantel et al., "Polymerization of murine macrophage inflammatory protein 1.alpha. inactivates its myelosuppressive effects in vitro: The active form is a monomer", Proc. Natl. Acad. Sci. USA, 90 :2232-2236 (1993).
Maze et al., "Myelosuppressive Effects In Vivo of Purified Recombinant Murine Macrophage Inflammatory Protein-1.alpha.", J. Immunol. 149 :1004-1009 (1992).
Moser et al., Interleukin-8 Antagonists Generated by N-terminal Modification,: J. Biol. Chem., 268 :7125-7128 (1993).
Oppenheim et al., "Properties of the Novel Proinflammatory Supergene `Intercrine` Cytokine Family", Ann. Rev. Immunol., 9 :617-648 (1991).
Rajarathram et al., "Neutrophil Activation of Monomeric Interleukin-8", Science, 264 :90-90 (1994).
Tekamp-Olson et al., "Cloning and Characterization of cDNAs for Murine Macrophage Inflammatory Protein 2 and its Human Homologues", J. Exp. Med., 172:911-919 (1990).
Wolpe et al., "Macrophage inflmmatory proteins 1 and 2: members of a novel superfamily of cytokines", FASEB, 3:2565-2573 (1989).
Bidlingmeyer et al., "Rapid Analysis of Amino Acids Using Pre-Column Derivatization" J. Chrom., 336 :93-104 (1984).
Smith et al., "Measurement of Protein Using Bicinchoninic Acid", Anal. Biochemistry, 150 :76-85 (1985).
Poncz et al., "Cloning and Characterization of Platelet Factor 4 cDNA Derived from a Human Erythroleukemic Cell Line", Blood, 69 :219-223 (1987).
Hebert et al., "Endothelial and Leukocyte Forms of IL-8; Conversion by Thrombin and Interactions with Neutrophils", J. Immunol., 145 :3033-3040 (1990).
Clark-Lewis et al., "Chemical Synthesis, Purification, and Characterization of Two Inflammatory Proteins, Neutrophil Activating Peptide 1 (Interleukin-8) and Neutrophil Activating Peptide 2", Biochemistry 30 :3128-3135 (1991).
Thomas et al., "The Interleukin-8 Receptor is Encoded by a Neutrophil-specific cDNA clone, F3R", J. Biol. Chem., 266 :14839-14841 (1991).
LaRosa et al., "Amino Terminus of the Interleukin-8 Receptor is a Major Determinant of Receptor Subtype Specificity", J. Biol. Chem., 267:25402-25406 (1992).
Caen et al., "Platelet Factor 4 (PF4) and a Dodecapeptide Related to PF4 Protect Hematopoietic Precursor Cells from Chemotherapy", Blood, 82 :162a, Abstract No. 635 (1993).
Hecht, Drug and Market Development, 4 (3):49 (1993).
Johnson et al., "Cloning of Two Rabbit GRO Homologues and Their Expression in Alveolar Macrophages", Gene, 151:337-338, (1994).
Power et al., "Cloning of a Full-Length cDNA Encoding the Neutrophil-activating Peptide ENA-78 from Human Platelets", Gene, 151:333-334, (1994).

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