Chemiluminescent reagent and assay using a substituted acetanili

Details Chemiluminescent reagent and assay using a substituted acetanili Chemiluminescent reagent and assay using a substituted acetanili

1994-08-29

1998-01-06

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

436172, 435 71, C12Q 128

Patent

active

057053570

ABSTRACT:
A simplified composition for generating a chemiluminescent signal in the presence of a peroxidase includes a substituted acetanilide as the sole light-producing substrate for the peroxidase. Assays for various analytes can be carried out with this composition at near neutral pH, exhibit low background and are highly sensitive.

REFERENCES:
patent: 5279940 (1994-01-01), Kissel
DeToledo et al, Anal. Biochem., 105, 36-38, 1980.
Slawinska et al, J. Biolum. Chemilum., 4, 226-230, 1989.
Segawa et al, Anal. Sciences, 6, 763-764, 1990.
Slawinska et al, J. Luminescence, 40 & 41, 262-266, 1988.
Koukli et al, Analyst, 114, 711-714, 1989.
K. Schmitt, et al., Photochem. Photobiol., "The Peroxidase-Promoted Metabolic Activation of Acetaminophen Produces Electronically Excited Species", vol. 51, No. 6, pp. 719-723, 1990.

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