Chemiluminescent immunoassays with triphenylmethane dyes activat

Details Chemiluminescent immunoassays with triphenylmethane dyes activat Chemiluminescent immunoassays with triphenylmethane dyes activat

1981-05-26

1984-02-21

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436536, 436546, 436800, 436820, G01N 3354, G01N 3358, G01N 3352

Patent

active

044330600

ABSTRACT:
Process for the quantitative and qualitative determination of antigens, antibodies and their complexes by means of a chemiluminescing labelling substance activated or excited to chemiluminescence by an analytical reagent. By means of a serological reaction, initially an antigen/antibody complex is formed which is treated with a chemiluminescing conjugate containing chemiluminescing triphenylmethane dyes and the chemiluminescence of the chemiluminescing complex formed is measured. Qualitative or quantitative details on the substances to be measured are obtained on the basis of the resulting measured values. The dye labels are activated by either hypochlorites or mixtures of H.sub.2 O.sub.2 and a chloramine.

REFERENCES:
patent: 4104029 (1978-08-01), Maier
patent: 4238195 (1980-12-01), Boguslaski

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