Chemiluminescent enzyme immunoassay

Details Chemiluminescent enzyme immunoassay Chemiluminescent enzyme immunoassay

1978-03-07

1981-11-24

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 435 8, 435291, 23230B, 424 8, 424 12, 422 52, G01N 3354, C12Q 168, C12M 134

Patent

active

043025340

ABSTRACT:
A heterogeneous enzymatic immunoassay, in which chemiluminescence is employed as a detection means, is disclosed. The chemiluminescence is produced by an enzymatic catalyzed redox reaction between hydrogen peroxide and a phenolic compound such as pyrogallol, resorcinol, phloroglucinol and hydroquinone. The assay is disclosed as having sensitivity equal to that of radioimmunoassays and reverse hemaglutination tests without some of the drawbacks of these methods, e.g., radioactivity hazards.

REFERENCES:
patent: 3359973 (1967-12-01), Hoffman
patent: 3575812 (1971-04-01), Chappelle
patent: 3791932 (1974-02-01), Schuurs et al.
patent: 3852157 (1974-12-01), Rubenstein et al.
patent: 3999948 (1976-12-01), Deindoerfer et al.
patent: 4101383 (1978-07-01), Wyatt et al.
patent: 4104029 (1978-08-01), Maier
patent: 4134792 (1979-01-01), Boguslaski et al.
Mahler, et al., Biological Chemistry, Harper and Row, New York, (1966), pp. 578-580 and 590-592.
Arakawa, et al., "Enzyme Immunoassay of Cortisol by Chemiluminescence of Luminol-Peroxidase", Banseki Kagaku, vol. 26 (1977).
O'Brien, et al., "Generation of Bio-Electronic Energy by Electron Transfer; Reduction of Peroxides Compound I and Compound II by Eosine", Biochem. Biophys. Res. Comm., vol. 81, No. 1, (1978), pp. 75-81.
Halmann, et al., "Peroxidase Mediated Chemiluminescence with Phenol Derivatives. Physicochemical Parameters and Uses in Biological Assays", Photochem. Photobiol., vol. 30 (1979), pp. 165-167.
Velian et al., "Chemiluminescence Immunoassay; A New Sensitive Method for Determination of Antigens" Immuno-Chemistry, vol. 15, (1978), pp. 331-333.
Velian et al., "Solid Phase Chemiluminescent Immunoassay" Proc. Int. Sym. Anal. App. Biolum. and Chemilum., (Brussels, 1978), pp. 431-437.

Affiliated with

Also associated with

Rating

Chemiluminescent enzyme immunoassay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chemiluminescent enzyme immunoassay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chemiluminescent enzyme immunoassay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2175404