Chemiluminescent energy transfer assays

Details Chemiluminescent energy transfer assays Chemiluminescent energy transfer assays

1997-03-04

1998-12-15

Green, Lora M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 74, 435 79, 435 792, 435 21, 435968, G01N 3353, G01N 35535

Patent

active

058494952

ABSTRACT:
A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos.TM. as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioassay for the presence or concentration of a biopolymer comprising a) an enzyme complex; b) a 1,2-dioxetane; and c) AttoPhos.TM..

REFERENCES:
patent: 5004565 (1991-04-01), Schaap
patent: 5112960 (1992-05-01), Bronstein et al.
R.J. Cano, et al., Detection of Salmonellas By DNA Hybridization With A Fluorescent Alkaline Phosphatase Substrate, Journal of Applied Bacteriology, 1992, 72:393-399.
Ramon A. Evangelista, et al., Alkyl-and Aryl-Substituted Salicyl Phosphates as Detection Reagents In Enzyme-Amplified Fluorescence DNA Hybridizatin Assays on Solid Support, Analytical Biochemistry 1992 203:218-226.
I. Bronstein, et al., Mproved Chemiluminescent Western Blotting Procedure, Product Application Focus, vol. 12, No. 5 (1992).

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