Chemiluminescent double-triggered 1, 2-dioxetanes

Details Chemiluminescent double-triggered 1, 2-dioxetanes Chemiluminescent double-triggered 1, 2-dioxetanes

1989-05-31

1992-07-21

Griffin, Ronald W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 772, 435 21, 435 28, 252700, 558 70, 549200, 549218, C07D31902, C12Q 100, C12Q 128, C12Q 142

Patent

active

051322048

ABSTRACT:
A stable 1,2-dioxetane of the formula: ##STR1## wherein X' is a phenyl group with 0 to 2 Cl or I atoms. This 1,2-dioxetane is a double-trigger substrate. The 2-methyl-4-hydroxy-naphthyl group is first removed by horseradish peroxidase. The exposed phosphate group is then removed with alkaline phosphatase. The exposed phenoxy 1,2-dioxetane decomposes to generate chemiluminescence output in immunoassays or nucleic acid hybridization assays.

REFERENCES:
patent: 3720622 (1973-03-01), Bollyky
patent: 4931223 (1990-06-01), Bronstein
patent: 4959182 (1990-09-01), Schaap
Schaap et al., (1987), Tetrahedron Letters, vol. 28, No. 11, pp. 1155-1158, 1159 -1162.
McCapra, (1968) Chemical Communications, pp. 155-156.
Kopecky et al., (1969) Canadian Journal of Chemistry 47:709-711.
Wieringa et al., (1972) Tetrahedron Letters 2:169-172.

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