Chemiluminescent detection of heme proteins in biological sample

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 436 66, C12Q 128, C12Q 100

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060871210

ABSTRACT:
Assays are disclosed for evaluating the presence or amount of a heme protein in a sample. The assays are based on chemiluminescence. Assay solutions include the sample, a suitable substrate and an oxidizing agent. The substrate is selected to undergo a specific reaction with the proteins of interest to produce an intermediate that emits light. suitable heme proteins include hemoglobin and myeloperoxidase. Suitable substrates include cyclic hydrazides and acridan derivatives.

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