Chemiluminescent detecting method and apparatus

Chemistry: analytical and immunological testing – Peptide – protein or amino acid

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430 56, 430 94, 430172, 430501, 422 52, 435 6, 2504844, 250581, 250582, 250583, 250584, 250585, 250586, G01N 3348, G01N 2176, G01N 2304

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056725140

ABSTRACT:
A chemiluminescent detecting method includes the steps of uniformly irradiating with radiation a stimulable phosphor sheet formed with a stimulable phosphor layer containing a stimulable phosphor which can store an energy of radiation and be stimulated by visible light to emit the energy of radiation in a form of light, thereby storing energy of radiation uniformly therein, selectively labeling a biopolymer with a labeling substance which can produce chemiluminescent light by contact of itself and a chemiluminescent substance, causing the biopolymer labeled with the labeling substance and the chemiluminescent substance to come into contact with each other, and exposing the stimulable phosphor sheet to chemiluminescent light produced by the contact of the biopolymer labeled with the labeling substance and the chemiluminescent substance. According to this chemiluminescent detecting method, it is possible to effectively produce information relating to a biopolymer such as information relating to a gene with high accuracy by using a stimulable phosphor sheet which can be easily handled and used for both the chemiluminescent detecting method and the autoradiographic detecting method.

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