Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1996-06-11
1999-08-10
Ceperley, Mary E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
435 6, 435805, 435815, 435818, 436544, 5303915, G01N 33531, G01N 33576, G01N 3376
Patent
active
059360704
ABSTRACT:
Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises providing (1) combining a medium suspected of containing the analyte and a novel chemiluminescent compound, (2) combining a means for chemically activating the chemiluminescent compound; and (3) detecting the amount of luminescence generated by the chemiluminescent compound. The amount of luminescence generated is related to the amount of analyte in the medium. The chemiluminescent compound can be chemically activated by hydrogen peroxide. Compositions and kits are also disclosed.
The chemiluminiscent compound is a spiro-acridan and has ##STR1## where X and Y are independently O, S, Se or NH; and Z is a chain 1-5 atoms in length; 0 to 8 hydrogens of the compound alone or taken together, may be replaced an alkyl, alkylidine, aryl aralkyl, or an alkyl, aryl or aralkyl substituted with one or more radicals of functional groups; 1 to 4 of the aromatic carbon atoms may be replaced by nitrogen atoms; and 0 to 1 hydrogens may be replaced by a specific binding pair member or fluorescent group.
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Meneghini Frank
Singh Rajendra
Singh Sharat
Ullman Edwin F.
Ceperley Mary E.
Dade Behring Marburg GmbH
Leitereg Theodore J
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